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验证数据展示

  • IHC staining of mouse heart using 19836-1-AP

    IHC staining of mouse heart using 19836-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 19836-1-AP (TWEAKR antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

TWEAKR/CD266 Polyclonal antibody

TWEAKR/CD266 Polyclonal Antibody for IHC, ELISA
Cat No. 19836-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

IHC, ELISA

别名

CD266, TNFRSF12A, TWEAKR, FGF-inducible 14, FGF inducible 14

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive IHC detected inmouse heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

19836-1-AP targets TWEAKR/CD266 in IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 TWEAKR/CD266 fusion protein Ag13823 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 tumor necrosis factor receptor superfamily, member 12A
别名 CD266, TNFRSF12A, TWEAKR, FGF-inducible 14, FGF inducible 14
计算分子量 129 aa, 14 kDa
GenBank蛋白编号BC002718
基因名称 TWEAKR
Gene ID (NCBI) 51330
RRIDAB_3669316
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9NP84
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TWEAKR (also known as CD266, FN14, TNFRSF12A) is a member of the TNF receptor superfamily which is activated by its ligand, the cytokine TWEAK (TNFSF12). TWEAKR is the smallest member of the TNFR superfamily. TweakR was first described as an FGF-inducible gene that played a role in fibroblast adhesion and migration. Subsequently, the induction of TweakR expression by other growth factors and/or upon tissue injury was observed in multiple cell types, including hepatocytes, endothelial cells, adipocytes, and cardiomyocytes. (PMID: 23073510, PMID: 24409185)

实验方案

Product Specific Protocols
IHC protocol for TWEAKR/CD266 antibody 19836-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols