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- KD/KO Validated
TWIST1-specific Polyclonal antibody
TWIST1-specific Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse and More (2)
Applications
WB, IHC, IF/ICC, IP, ELISA and More (3)
Conjugate
Unconjugated
166
验证数据展示
经过测试的应用
Positive WB detected in | U-251 cells, Raji cells, A549 cells, SH-SY5Y cells, MDA-MB-231 cells, PC-3 cells |
Positive IP detected in | MDA-MB-453s cells |
Positive IHC detected in | human liver cancer tissue, human breast cancer tissue, human pancreas cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | L02 cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:4000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:300-1:1200 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
25465-1-AP targets TWIST1-specific in WB, IHC, IF/ICC, IP, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse samples.
Tested Applications | WB, IHC, IF/ICC, IP, ELISA Application Description |
Cited Applications | WB, IHC, IF, IP, CoIP, ChIP |
Tested Reactivity | human, mouse |
Cited Reactivity | human, mouse, rat, zebrafish |
Immunogen | TWIST1-specific fusion protein Ag21924 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | twist homolog 1 (Drosophila) |
Synonyms | ACS3, bHLHa38, BPES2, BPES3, H twist, SCS, TWIST, twist homolog 1 (Drosophila), Twist related protein 1, TWIST1, TWIST1-specific |
Calculated Molecular Weight | 202 aa, 21 kDa |
Observed Molecular Weight | 25-30 kDa |
GenBank Accession Number | BC036704 |
Gene Symbol | TWIST1 |
Gene ID (NCBI) | 7291 |
RRID | AB_2880093 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | Q15672 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
---|---|
WB protocol for TWIST1-specific antibody 25465-1-AP | Download protocol |
IHC protocol for TWIST1-specific antibody 25465-1-AP | Download protocol |
IF protocol for TWIST1-specific antibody 25465-1-AP | Download protocol |
IP protocol for TWIST1-specific antibody 25465-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Sci Adv Phospholipid remodeling is critical for stem cell pluripotency by facilitating mesenchymal-to-epithelial transition. | ||
J Exp Clin Cancer Res ACTN1 promotes HNSCC tumorigenesis and cisplatin resistance by enhancing MYH9-dependent degradation of GSK-3β and integrin β1-mediated phosphorylation of FAK | ||
Int J Biol Macromol Fucoidan/chitosan layered PLGA nanoparticles with melatonin loading for inducing intestinal absorption and addressing triple-negative breast cancer progression | ||
Int J Nanomedicine Combined miR-181a-5p and Ag Nanoparticles are Effective Against Oral Cancer in a Mouse Model | ||
Clin Transl Med FABP4 deactivates NF-κB-IL1α pathway by ubiquitinating ATPB in tumor-associated macrophages and promotes neuroblastoma progression. | ||
Clin Transl Med Aptamer-SH2 superbinder-based targeted therapy for pancreatic ductal adenocarcinoma. |