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TWIST2 Monoclonal antibody, PBS Only

TWIST2 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, rat, mouse, pig

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1B12D11

Cat no : 66544-1-PBS

Print datasheet

Synonyms

bHLHa39, Dermis expressed protein 1, Dermo 1, DERMO1, twist homolog 2 (Drosophila), Twist related protein 2, TWIST2

验证数据展示

  • WB analysis using 66544-1-Ig (same clone as 66544-1-PBS)

    WB analysis using 66544-1-Ig (same clone as 66544-1-PBS)

    Various cells were subjected to SDS PAGE followed by western blot with 66544-1-Ig (TWIST2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66544-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 66544-1-Ig (same clone as 66544-1-PBS)

    IHC staining of human breast cancer using 66544-1-Ig (same clone as 66544-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66544-1-Ig (TWIST2 antibody) at dilution of 1:500 (under 40x lens). This data was developed using the same antibody clone with 66544-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with TWIST2 antibody included
  • IF Staining of HepG2 using 66544-1-Ig (same clone as 66544-1-PBS)

    IF Staining of HepG2 using 66544-1-Ig (same clone as 66544-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using TWIST2 antibody (66544-1-Ig, Clone: 1B12D11 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66544-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy TWIST2 Ready-To-Use IHC Kit. TWIST2 primary antibody included.

产品信息

66544-1-PBS targets TWIST2 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human, rat, mouse, pig samples.

Tested Applications WB, IHC, IF/ICC, Indirect ELISA Application Description
Tested Reactivity Human, rat, mouse, pig
Immunogen TWIST2 fusion protein Ag2348 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name twist homolog 2 (Drosophila)
Synonyms bHLHa39, Dermis expressed protein 1, Dermo 1, DERMO1, twist homolog 2 (Drosophila), Twist related protein 2, TWIST2
Calculated Molecular Weight 160 aa, 18 kDa
Observed Molecular Weight 22 kDa
GenBank Accession NumberBC033168
Gene Symbol TWIST2
Gene ID (NCBI) 117581
RRIDAB_2881906
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ8WVJ9
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

TWIST2, also named as BHLHA39 and DERMO1,is a basic helix-loop-helix protein which acts as a transcriptional regulator, plays a central role in differentiation of mesodermal during embryogenesis. TWIST2 inhibits the premature or ectopic differentiation of preosteoblast cells during osteogenesis, possibly by changing the internal signal transduction response of osteoblasts to external growth factors. It is also implicated in tumorigenesis, invasion and metastasis. TWIST2 forms both homodimers(45KD) and heterodimers with E2A E proteins, and dimer partner selection is a critical mediator of TWIST function. (PMID:14724576, 16502419).