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TWISTNB Polyclonal antibody

TWISTNB Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 22784-1-AP

Print datasheet

Synonyms

TWIST neighbor, RPA43, RNA polymerase I subunit F, POLR1F, DNA-directed RNA polymerase I subunit RPA43

验证数据展示

  • WB analysis of THP-1 using 22784-1-AP

    WB analysis of THP-1 using 22784-1-AP

    THP-1 cells were subjected to SDS PAGE followed by western blot with 22784-1-AP (TWISTNB antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • WB analysis using 22784-1-AP

    WB analysis using 22784-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 22784-1-AP (TWISTNB antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of human ovary cancer using 22784-1-AP

    IHC staining of human ovary cancer using 22784-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 22784-1-AP (TWISTNB antibody) at dilution of 1:600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells, THP-1 cells
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:300-1:1200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

22784-1-AP targets TWISTNB in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human
Immunogen TWISTNB fusion protein Ag18783 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name TWIST neighbor
Synonyms TWIST neighbor, RPA43, RNA polymerase I subunit F, POLR1F, DNA-directed RNA polymerase I subunit RPA43
Calculated Molecular Weight 338 aa, 37 kDa
Observed Molecular Weight 40-50 kDa
GenBank Accession NumberBC130298
Gene Symbol TWISTNB
Gene ID (NCBI) 221830
RRIDAB_2879167
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ3B726
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for TWISTNB antibody 22784-1-APDownload protocol
IHC protocol for TWISTNB antibody 22784-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols