验证数据展示
经过测试的应用
| Positive IHC detected in | human lymphoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | Hela cells, HepG2 cells |
Planning an IHC experiment? We recommend our IHCeasy TXK Ready-To-Use IHC Kit. TXK primary antibody included.
推荐稀释比
| Application | Dilution |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| IHC | See 1 publications below |
产品信息
18981-1-AP targets TXK in IHC, IF/ICC, ELISA applications and shows reactivity with human samples.
| Tested Applications | IHC, IF/ICC, ELISA Application Description |
| Cited Applications | IHC |
| Tested Reactivity | human |
| Cited Reactivity | human |
| Immunogen | Peptide 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | TXK tyrosine kinase |
| Synonyms | PTK4, PSCTK5, Protein-tyrosine kinase 4, Protein tyrosine kinase 4, EC:2.7.10.2 |
| Calculated Molecular Weight | 61 kDa |
| GenBank Accession Number | NM_003328 |
| Gene Symbol | TXK |
| Gene ID (NCBI) | 7294 |
| RRID | AB_10598482 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P42681 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
TXK, also named as PTK4, is an enzyme that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation.
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for TXK antibody 18981-1-AP | Download protocol |
| IF protocol for TXK antibody 18981-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
