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验证数据展示

  • WB analysis using 27558-1-AP

    WB analysis using 27558-1-AP

    Various cells were subjected to SDS PAGE followed by western blot with 27558-1-AP (TXLNA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human ovary cancer using 27558-1-AP

    IHC staining of human ovary cancer using 27558-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 27558-1-AP (Alpha Taxilin antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Featured Product
  • KD/KO验证

Alpha Taxilin Polyclonal antibody

Alpha Taxilin Polyclonal Antibody for WB, IHC, ELISA
Cat No. 27558-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA and More (1)

别名

TXLNA, TXLN, taxilin alpha, RP4 622L5.4, IL14

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells, HepG2 cells, Jurkat cells
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27558-1-AP targets Alpha Taxilin in WB, IHC, IF, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB, IF
经测试反应性 human
文献引用反应性human
免疫原 Alpha Taxilin fusion protein Ag23001 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 taxilin alpha
别名 TXLNA, TXLN, taxilin alpha, RP4 622L5.4, IL14
计算分子量 546 aa, 62 kDa
观测分子量 75 kDa
GenBank蛋白编号BC103823
基因名称 Alpha Taxilin
Gene ID (NCBI) 200081
RRIDAB_2880907
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP40222
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Alpha-taxilin is a member of the taxilin family which consist of at least three members: alpha-, beta- and gamma-taxilins. Alpha-taxilin was identified as a novel binding partner of the syntaxin family, which is involved in intracellular vesicle traffic. Alpha-taxilin is ubiquitously expressed. It plays an essential role for release of HBV-DNA containing particles. Alpha-taxilin encompasses 546 aa and has a calculated molecular weight of 62 kDa. It migrates on SDS-PAGE with an apparent molecular weight of 75 kDa. (PMID: 12558796; 23816704)

实验方案

Product Specific Protocols
WB protocol for Alpha Taxilin antibody 27558-1-APDownload protocol
IHC protocol for Alpha Taxilin antibody 27558-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IF

Elife

α-/γ-Taxilin are required for centriolar subdistal appendage assembly and microtubule organization.

Authors - Dandan Ma
  • KD Validated
View Article