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TXNDC11 Polyclonal antibody

TXNDC11 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 25687-1-AP

Print datasheet

Synonyms

Thioredoxin domain-containing protein 11, EFP1, EF-hand-binding protein 1, EF hand binding protein 1

验证数据展示

  • WB analysis using 25687-1-AP

    WB analysis using 25687-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 25687-1-AP (TXNDC11 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of HEK-293 using 25687-1-AP

    WB analysis of HEK-293 using 25687-1-AP

    WB result of TXNDC11 antibody (25687-1-AP; 1:400; incubated at room temperature for 1.5 hours) with sh-Control and sh-TXNDC11 transfected HEK-293 cells.

  • IHC staining of human intrahepatic cholangiocarcinoma using 25687-1-AP

    IHC staining of human intrahepatic cholangiocarcinoma using 25687-1-AP

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 25687-1-AP (TXNDC11 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human intrahepatic cholangiocarcinoma using 25687-1-AP

    IHC staining of human intrahepatic cholangiocarcinoma using 25687-1-AP

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 25687-1-AP (TXNDC11 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inPC-3 cells, HEK-293 cells
Positive IHC detected inhuman intrahepatic cholangiocarcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25687-1-AP targets TXNDC11 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human
Immunogen TXNDC11 fusion protein Ag22618 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name thioredoxin domain containing 11
Synonyms Thioredoxin domain-containing protein 11, EFP1, EF-hand-binding protein 1, EF hand binding protein 1
Observed Molecular Weight 100-110 kDa
GenBank Accession NumberBC013727
Gene Symbol TXNDC11
Gene ID (NCBI) 51061
RRIDAB_2880195
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ6PKC3
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for TXNDC11 antibody 25687-1-APDownload protocol
IHC protocol for TXNDC11 antibody 25687-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols