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验证数据展示

  • WB analysis of human brain using 20181-1-AP

    WB analysis of human brain using 20181-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 20181-1-AP (ERp19 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver cancer using 20181-1-AP

    IHC staining of human liver cancer using 20181-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 20181-1-AP (ERp19 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with TXNDC12 antibody included
  • IHC staining of human liver cancer using 20181-1-AP

    IHC staining of human liver cancer using 20181-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 20181-1-AP (ERp19 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with TXNDC12 antibody included

ERp19 Polyclonal antibody

ERp19 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 20181-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

AGR1, AIF, AIFM1, ERp18, ERp19, hAG 1, PDCD8, PDIA16, TLP19, TXNDC12

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inhuman brain tissue, human heart tissue, mouse kidney tissue, mouse lung tissue
Positive IHC detected inhuman liver cancer tissue, human hepatocirrhosis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy TXNDC12 Ready-To-Use IHC Kit. TXNDC12 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

20181-1-AP targets ERp19 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 ERp19 fusion protein Ag14045 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 thioredoxin domain containing 12 (endoplasmic reticulum)
别名 AGR1, AIF, AIFM1, ERp18, ERp19, hAG 1, PDCD8, PDIA16, TLP19, TXNDC12
计算分子量 172 aa, 19 kDa
观测分子量 20 kDa, 40 kDa
GenBank蛋白编号BC001493
基因名称 TXNDC12
Gene ID (NCBI) 51060
RRIDAB_10646468
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO95881
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TXNDC12, which is known as thioredoxin domain-containing protein 12, ERp16, ERp18, ERp19, or hTLP19, is a member of the protein disulfide isomerase (PDI) family that plays important roles in cancer development and progression. TXNDC12 has been demonstrated to contribute to tumorigenicity in human gastric cancer by promoting cell growth, migration, and invasion(PMID: 25940440). Other studies also revealed the overexpression of TXNDC12 promotes HCC metastasis through β-catenin-mediated induction of EMT and that TXNDC12 has the potential to be a prognostic factor and therapeutic target of HCC. (PMID: 31570854)

实验方案

Product Specific Protocols
WB protocol for ERp19 antibody 20181-1-APDownload protocol
IHC protocol for ERp19 antibody 20181-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols