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TXNDC17 Polyclonal antibody

TXNDC17 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 20811-1-AP

Print datasheet

Synonyms

TXNL5, TRP14, Thioredoxin-like protein 5, Thioredoxin domain-containing protein 17, Protein 42-9-9

验证数据展示

  • WB analysis using 20811-1-AP

    WB analysis using 20811-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 20811-1-AP (TXNDC17 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

  • WB analysis of Jurkat using 20811-1-AP

    WB analysis of Jurkat using 20811-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 20811-1-AP (TXNDC17 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 20811-1-AP

    IF Staining of HepG2 using 20811-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using TXNDC17 antibody (20811-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

  • IP experiment of HEK-293 using 20811-1-AP

    IP experiment of HEK-293 using 20811-1-AP

    IP result of anti-TXNDC17 (IP:20811-1-AP, 4ug; Detection:20811-1-AP 1:6000) with HEK-293 cells lysate 1575 ug.

  • IF Staining of HeLa using 20811-1-AP

    IF Staining of HeLa using 20811-1-AP

    Immunofluorescent analysis of Hela cells, using TXNDC17 antibody 20811-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

经过测试的应用

Positive WB detected inA549 cells, HEK-293 cells, HeLa cells, human kidney tissue, human uterus tissue, Jurkat cells, K-562 cells, L02 cells, mouse pancreas tissue
Positive IP detected inHEK-293 cells
Positive IF/ICC detected inHepG2 cells, HeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

20811-1-AP targets TXNDC17 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IF/ICC, IP, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Immunogen TXNDC17 fusion protein Ag14766 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name thioredoxin domain containing 17
Synonyms TXNL5, TRP14, Thioredoxin-like protein 5, Thioredoxin domain-containing protein 17, Protein 42-9-9
Calculated Molecular Weight 123 aa, 14 kDa
Observed Molecular Weight 14 kDa
GenBank Accession NumberBC006405
Gene Symbol TXNDC17
Gene ID (NCBI) 84817
RRIDAB_10695499
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BRA2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TXNDC17 also known as TRP14, TXNL5, belongs to the thioredoxin family. TRP14 is a widely expressed, 123-amino acid protein with a WCPDC motif. TXNDC17 has a thioredoxin fold and a TRX-like active-site sequence(PMID: 14607844, 24778250).

实验方案

Product Specific Protocols
WB protocol for TXNDC17 antibody 20811-1-APDownload protocol
IF protocol for TXNDC17 antibody 20811-1-APDownload protocol
IP protocol for TXNDC17 antibody 20811-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Int J Radiat Biol

Combination of podophyllotoxin and rutin modulate radiation-induced alterations of jejunal proteome in mice.

Authors - Sania Bajaj
View Article