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验证数据展示

  • WB analysis of HeLa using 25208-1-AP

    WB analysis of HeLa using 25208-1-AP

    WB result of TXNDC9 antibody (25208-1-AP; 1:15000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TXNDC9 transfected HeLa cells.

  • WB analysis of Raji using 25208-1-AP

    WB analysis of Raji using 25208-1-AP

    Raji cells were subjected to SDS PAGE followed by western blot with 25208-1-AP (TXNDC9 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of HEK-293 using 25208-1-AP

    IF Staining of HEK-293 using 25208-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HEK-293 cells using 25208-1-AP (TXNDC9 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

  • Featured Product
  • KD/KO验证

TXNDC9 Polyclonal antibody

TXNDC9 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 25208-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

APACD, PHLP3, Protein 1 4, TXNDC9

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inRaji cells, HEK-293 cells, K-562 cells, HeLa cells
Positive IF/ICC detected inHEK-293 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25208-1-AP targets TXNDC9 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 TXNDC9 fusion protein Ag19509 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 thioredoxin domain containing 9
别名 APACD, PHLP3, Protein 1 4, TXNDC9
计算分子量 226 aa, 27 kDa
观测分子量 25-27 kDa
GenBank蛋白编号BC005968
基因名称 TXNDC9
Gene ID (NCBI) 10190
RRIDAB_2879961
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO14530
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TXNDC9, also known as APACD, has a negative effect on protein folding by significantly reducing the activity of chaperone protein TCP1 complex ATPase activity. Including actin or tubulin.

实验方案

Product Specific Protocols
WB protocol for TXNDC9 antibody 25208-1-APDownload protocol
IF protocol for TXNDC9 antibody 25208-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols