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验证数据展示

  • WB analysis using 27429-1-AP

    WB analysis using 27429-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27429-1-AP (TXNIP antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse colon using 27429-1-AP

    IHC staining of mouse colon using 27429-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 27429-1-AP (TXNIP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse colon using 27429-1-AP

    IHC staining of mouse colon using 27429-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 27429-1-AP (TXNIP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HeLa using 27429-1-AP

    IF Staining of HeLa using 27429-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using TXNIP antibody (27429-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

TXNIP Polyclonal antibody

TXNIP Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 27429-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, ELISA

别名

EST01027, HHCPA78, THIF, Thioredoxin binding protein 2, TXNIP, VDUP1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, MCF-7 cells, RAW 264.7 cells
Positive IHC detected inmouse colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27429-1-AP targets TXNIP in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse
免疫原 TXNIP fusion protein Ag26579 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 thioredoxin interacting protein
别名 EST01027, HHCPA78, THIF, Thioredoxin binding protein 2, TXNIP, VDUP1
计算分子量 391 aa, 44 kDa
观测分子量 50-55 kDa
GenBank蛋白编号BC093702
基因名称 TXNIP
Gene ID (NCBI) 10628
RRIDAB_3085957
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9H3M7
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TXNIP, also known as VDUP-1 or TBP-2, belongs to alpha-arrestin protein family and is perhaps the only family member known to bind thioredoxin (TRX). TXNIP was induced by Vitamin D3, but not induced by another monocyte or macrophage differentiation inducer: phorbol 12-myristate 13-acetate (PMA). TXNIP bound catalytic active center of thioredoxin (TRX), which protected cells against oxidative stress. TXNIP was found to be a negative regulator of thioredoxin activity and inducer of the intracellular level of reactive oxygen species (ROS). TXNIP plays an important role in a wide variety of biological functions, such as the regulation of cell death, growth, differentiation, and energy metabolism.

实验方案

Product Specific Protocols
WB protocol for TXNIP antibody 27429-1-APDownload protocol
IHC protocol for TXNIP antibody 27429-1-APDownload protocol
IF protocol for TXNIP antibody 27429-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols