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TXNL4A Polyclonal antibody

TXNL4A Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human and More (1)

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 27646-1-AP

Print datasheet

Synonyms

DIB1, DIM1, DIM1 protein homolog, HsT161, thioredoxin like 4A, Thioredoxin like protein 4A, TXNL4, TXNL4A, U5 15kD

验证数据展示

  • WB analysis of HEK-293 using 27646-1-AP

    WB analysis of HEK-293 using 27646-1-AP

    WB result of TXNL4A antibody (27646-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TXNL4A transfected HEK-293 cells.

  • WB analysis using 27646-1-AP

    WB analysis using 27646-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27646-1-AP (TXNL4A antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 27646-1-AP

    IF Staining of HepG2 using 27646-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 27646-1-AP (TXNL4A antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inHEK-293 cells, PC-3 cells, L02 cells, HepG2 cells
Positive IF/ICC detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

27646-1-AP targets TXNL4A in WB, IF/ICC, ELISA applications and shows reactivity with Human samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity Human
Cited Reactivityhuman
Immunogen TXNL4A fusion protein Ag26473 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name thioredoxin-like 4A
Synonyms DIB1, DIM1, DIM1 protein homolog, HsT161, thioredoxin like 4A, Thioredoxin like protein 4A, TXNL4, TXNL4A, U5 15kD
Calculated Molecular Weight 142 aa, 17 kDa
Observed Molecular Weight 14-20 kDa
GenBank Accession NumberBC001046
Gene Symbol TXNL4A
Gene ID (NCBI) 10907
RRIDAB_2880937
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP83876
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for TXNL4A antibody 27646-1-APDownload protocol
IF protocol for TXNL4A antibody 27646-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Nat Struct Mol Biol

Structure of the human 20S U5 snRNP

Authors - Sarah Schneider
View Article