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  • Featured Product
  • KD/KO Validated

TXNRD1 Monoclonal antibody, PBS Only

TXNRD1 Monoclonal Antibody for WB,Indirect ELISA,IHC,IF

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB,Indirect ELISA,IHC,IF

Conjugate

Unconjugated

CloneNo.

1B10C4

Cat No : 67728-1-PBS

Print datasheet

Synonyms

GRIM 12, GRIM12, KDRF, thioredoxin reductase 1, Thioredoxin reductase TR1, TR, TR1, TRXR1, TXNR, TXNRD1

验证数据展示

  • WB analysis using 67728-1-Ig (same clone as 67728-1-PBS)

    WB analysis using 67728-1-Ig (same clone as 67728-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67728-1-Ig (TXNRD1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67728-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 67728-1-Ig (same clone as 67728-1-PBS)

    IHC staining of human liver cancer using 67728-1-Ig (same clone as 67728-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67728-1-Ig (TXNRD1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67728-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with TXNRD1 antibody included
  • IF Staining of HeLa using 67728-1-Ig (same clone as 67728-1-PBS)

    IF Staining of HeLa using 67728-1-Ig (same clone as 67728-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using TXNRD1 antibody (67728-1-Ig, Clone: 1B10C4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 67728-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy TXNRD1 Ready-To-Use IHC Kit. TXNRD1 primary antibody included.

产品信息

67728-1-PBS targets TXNRD1 in WB, IF, IHC, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications WB,Indirect ELISA,IHC,IF Application Description
Tested Reactivity Human, mouse, rat
Immunogen TXNRD1 fusion protein Ag30071 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name thioredoxin reductase 1
Synonyms GRIM 12, GRIM12, KDRF, thioredoxin reductase 1, Thioredoxin reductase TR1, TR, TR1, TRXR1, TXNR, TXNRD1
Calculated Molecular Weight 55 kDa
Observed Molecular Weight50-70 kDa
GenBank Accession NumberBC018122
Gene Symbol TXNRD1
Gene ID (NCBI) 7296
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ16881
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

背景介绍

Thioredoxin reductase-1 (TXNRD1) is uniquely capable of utilizing electrons from NADPH to recover the reduced state of TRX1. Interestingly, TXNRD1 is upregulated in many human malignancies and promotes cancer progression, and attenuation of TXNRD1 levels effectively suppresses the growth of tumor cells (PMID: 31384178). TXNRD1 is also upregulated in many human malignancies and functions as a prognostic factor for many tumors, such as oral squamous cell carcinomas, lung cancer, breast cancer, and astrocytomas. TXNRD1 protein levels were analyzed by inmmunoblotting. Bands of ~55 kDa for TXNRD1 were observed. IHC analysis suggested TXNRD1 was mainly located in the cytoplasm. (PMID: 28536696, PMID: 20584310)