TXNRD2 Polyclonal antibody

TXNRD2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 16360-1-AP

Print datasheet

Synonyms

KIAA1652, Selenoprotein Z, SELZ, thioredoxin reductase 2, Thioredoxin reductase TR3, TR, TR BETA, TR3, TRXR2, TXNRD2



经过测试的应用

Positive WB detected inHepG2 cells, rat liver tissue, MCF-7 cells, HeLa cells
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16360-1-AP targets TXNRD2 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB, IHC, IF
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, pig
Immunogen TXNRD2 fusion protein Ag8367 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name thioredoxin reductase 2
Synonyms KIAA1652, Selenoprotein Z, SELZ, thioredoxin reductase 2, Thioredoxin reductase TR3, TR, TR BETA, TR3, TRXR2, TXNRD2
Calculated Molecular Weight 524 aa, 56 kDa
Observed Molecular Weight 54 kDa
GenBank Accession NumberBC007489
Gene Symbol TXNRD2
Gene ID (NCBI) 10587
RRIDAB_2210258
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NNW7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Human mitochondrial thioredoxin reductase (TXNRD2) is a selenocysteine-containing enzyme essential for mitochondrial oxygen radical scavenging. Three mammalian thioredoxin reductases exist; a cytosolic (TXNRD1), a mitochondrial (TXNRD2), and a testis-specific thioredoxin reductase (TXNRD3). (PMID: 21247928, PMID: 26199228)

实验方案

Product Specific Protocols
WB protocol for TXNRD2 antibody 16360-1-APDownload protocol
IHC protocol for TXNRD2 antibody 16360-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Adv Sci (Weinh)

Identification of PRDX5 as A Target for The Treatment of Castration-Resistant Prostate Cancer

Authors - Rong Wang
humanWB

Nat Chem Biol

Ribosome stalling during selenoprotein translation exposes a ferroptosis vulnerability.

Authors - Zhipeng Li
ratWB,IF

Oxid Med Cell Longev

Paeoniflorin Upregulates Mitochondrial Thioredoxin of Schwann Cells to Improve Diabetic Peripheral Neuropathy Indicated by 4D Label-Free Quantitative Proteomics.

Authors - Xinwei Yang
mouseWB

Antioxidants (Basel)

Selenium Status in Diet Affects Nephrotoxicity Induced by Cisplatin in Mice.

Authors - Shuang Liu
mouseWB

J Biol Chem

Redox regulation by TXNRD3 during epididymal maturation underlies capacitation-associated mitochondrial activity and sperm motility in mice.

Authors - Huafeng Wang
mouseWB

Sci Rep

Selenocysteine insertion sequence binding protein 2 (Sbp2) in the sex-specific regulation of selenoprotein gene expression in mouse pancreatic islets.

Authors - B Chellan