验证数据展示
经过测试的应用
| Positive WB detected in | HepG2 cells, rat liver tissue, MCF-7 cells, HeLa cells |
| Positive IHC detected in | human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
16360-1-AP targets TXNRD2 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat, pig |
| 免疫原 | TXNRD2 fusion protein Ag8367 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | thioredoxin reductase 2 |
| 别名 | KIAA1652, Selenoprotein Z, SELZ, thioredoxin reductase 2, Thioredoxin reductase TR3, TR, TR BETA, TR3, TRXR2, TXNRD2 |
| 计算分子量 | 524 aa, 56 kDa |
| 观测分子量 | 54 kDa |
| GenBank蛋白编号 | BC007489 |
| 基因名称 | TXNRD2 |
| Gene ID (NCBI) | 10587 |
| RRID | AB_2210258 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9NNW7 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Human mitochondrial thioredoxin reductase (TXNRD2) is a selenocysteine-containing enzyme essential for mitochondrial oxygen radical scavenging. Three mammalian thioredoxin reductases exist; a cytosolic (TXNRD1), a mitochondrial (TXNRD2), and a testis-specific thioredoxin reductase (TXNRD3). (PMID: 21247928, PMID: 26199228)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for TXNRD2 antibody 16360-1-AP | Download protocol |
| IHC protocol for TXNRD2 antibody 16360-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Adv Sci (Weinh) Identification of PRDX5 as A Target for The Treatment of Castration-Resistant Prostate Cancer | ||
Nat Chem Biol Ribosome stalling during selenoprotein translation exposes a ferroptosis vulnerability. | ||
Oxid Med Cell Longev Paeoniflorin Upregulates Mitochondrial Thioredoxin of Schwann Cells to Improve Diabetic Peripheral Neuropathy Indicated by 4D Label-Free Quantitative Proteomics. | ||
Antioxidants (Basel) Selenium Status in Diet Affects Nephrotoxicity Induced by Cisplatin in Mice. | ||
J Biol Chem Redox regulation by TXNRD3 during epididymal maturation underlies capacitation-associated mitochondrial activity and sperm motility in mice. | ||
Sci Rep Selenocysteine insertion sequence binding protein 2 (Sbp2) in the sex-specific regulation of selenoprotein gene expression in mouse pancreatic islets. |
