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TYK2 Recombinant antibody

TYK2 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

230176E7

Cat no : 83161-4-RR

Print datasheet

Synonyms

tyrosine kinase 2, JTK1, EC:2.7.10.2, 230176E7

验证数据展示

  • WB analysis using 83161-4-RR

    WB analysis using 83161-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83161-4-RR (TYK2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 83161-4-RR

    IF Staining of HepG2 using 83161-4-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using TYK2 antibody (83161-4-RR, Clone: 230176E7 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • FC experiment of U2OS using 83161-4-RR

    FC experiment of U2OS using 83161-4-RR

    1x10^6 U2OS cells were intracellularly stained with 0.25 ug TYK2 Recombinant antibody (83161-4-RR, Clone:230176E7) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • IHC staining of human ovary cancer using 83161-4-RR

    IHC staining of human ovary cancer using 83161-4-RR

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 83161-4-RR (TYK2 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with TYK2 antibody included
  • Affinity and Kinetic Characterization of 83161-4-RR

    Affinity and Kinetic Characterization of 83161-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83161-4-RR against Human TYK2 were performed. The affinity constant is 0.116 nM.

经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inU2OS cells
Planning an IHC experiment? We recommend our IHCeasy TYK2 Ready-To-Use IHC Kit. TYK2 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 83161-4-RR is TYK2 Fusion Protein expressed in E. coli.

Tested Applications WB, IHC, IF/ICC, FC (Intra), ELISA Application Description
Tested Reactivity human
Immunogen TYK2 fusion protein Ag28457 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name tyrosine kinase 2
Synonyms tyrosine kinase 2, JTK1, EC:2.7.10.2, 230176E7
Calculated Molecular Weight 1187 aa, 134 kDa
Observed Molecular Weight 134 kDa
GenBank Accession NumberBC014243
Gene Symbol TYK2
Gene ID (NCBI) 7297
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP29597
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for TYK2 antibody 83161-4-RRDownload protocol
IHC protocol for TYK2 antibody 83161-4-RRDownload protocol
IF protocol for TYK2 antibody 83161-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols