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Talin-1 Recombinant antibody, PBS Only

Talin-1 Recombinant Antibody for WB, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse, Rat

Applications

WB, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

7D11

Cat No : 82856-4-PBS

Print datasheet

Synonyms

TLN1, TLN, talin 1, KIAA1027, ILWEQ

验证数据展示

  • WB analysis using 82856-4-RR (same clone as 82856-4-PBS)

    WB analysis using 82856-4-RR (same clone as 82856-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82856-4-RR (Talin-1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82856-4-PBS in a different storage buffer formulation.

  • IF Staining of A549 using 82856-4-RR (same clone as 82856-4-PBS)

    IF Staining of A549 using 82856-4-RR (same clone as 82856-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using Talin-1 antibody (82856-4-RR, Clone: 7D11 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 82856-4-PBS in a different storage buffer formulation.

  • IF Staining of A549 using 82856-4-RR (same clone as 82856-4-PBS)

    IF Staining of A549 using 82856-4-RR (same clone as 82856-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using Talin-1 antibody (82856-4-RR, Clone: 7D11 ) at dilution of 1:1200 and Multi-rAb CoraLite ® Plus 594-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR004).(CL488-Phalloidin, green). This data was developed using the same antibody clone with 82856-4-PBS in a different storage buffer formulation.

  • IF Staining of A549 using 82856-4-RR (same clone as 82856-4-PBS)

    IF Staining of A549 using 82856-4-RR (same clone as 82856-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using Talin-1 antibody (82856-4-RR, Clone: 7D11 ) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red). This data was developed using the same antibody clone with 82856-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82856-4-PBS

    Affinity and Kinetic Characterization of 82856-4-PBS

    Biolayer interferometry (BLl) kinetic assays of 82856-4-RR against Human TLN1 were performed. The affinity constant is 0.215 nM.

产品信息

82856-4-PBS targets Talin-1 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Applications WB, IF/ICC, Indirect ELISA Application Description
Tested Reactivity Human, Mouse, Rat
Immunogen Talin-1 fusion protein Ag5367 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name talin 1
Synonyms TLN1, TLN, talin 1, KIAA1027, ILWEQ
Calculated Molecular Weight 2541 aa, 270 kDa
Observed Molecular Weight270 kDa
GenBank Accession NumberBC042923
Gene Symbol TLN1
Gene ID (NCBI) 7094
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9Y490
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Talin-1 is a large cytoskeletal protein consisting of a globular head domain and a long rod comprised of a series of α-helical bundles (PMID: 35266014). Talin-1 is ubiquitously expressed and is a major component of focal adhesions which are multi-protein, integrin-containing complexes, mediating cell migration, adhesion and integrin signalling (PMID: 33431906). Talin-1 also plays a role in tumor formation, migration and metastasis in various malignancies (PMID: 37013489).