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Tim23 Recombinant antibody, PBS Only

Tim23 Recombinant Antibody for WB,IP,Indirect ELISA,IHC,IF

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

WB,IP,Indirect ELISA,IHC,IF

Conjugate

Unconjugated

CloneNo.

1H16

Cat No : 82540-1-PBS

Print datasheet

Synonyms

PRO1197, TIM23, TIMM23

验证数据展示

  • WB analysis using 82540-1-RR (same clone as 82540-1-PBS)

    WB analysis using 82540-1-RR (same clone as 82540-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82540-1-RR (Tim23 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82540-1-PBS in a different storage buffer formulation.

  • IP experiment of HeLa using 82540-1-RR (same clone as 82540-1-PBS)

    IP experiment of HeLa using 82540-1-RR (same clone as 82540-1-PBS)

    IP result of anti-Tim23 (IP:82540-1-RR, 4ug; Detection:82540-1-RR 1:1000) with HeLa cells lysate 1280 ug. This data was developed using the same antibody clone with 82540-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse heart using 82540-1-RR (same clone as 82540-1-PBS)

    IHC staining of mouse heart using 82540-1-RR (same clone as 82540-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 82540-1-RR (Tim23 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82540-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 82540-1-RR (same clone as 82540-1-PBS)

    IF Staining of HepG2 using 82540-1-RR (same clone as 82540-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Tim23 antibody (82540-1-RR, Clone: 1H16 ) at dilution of 1:500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 82540-1-PBS in a different storage buffer formulation.

产品信息

82540-1-PBS targets Tim23 in WB, IP, IF, IHC, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications WB,IP,Indirect ELISA,IHC,IF Application Description
Tested Reactivity Human, mouse, rat
Immunogen Tim23 fusion protein Ag1620 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name translocase of inner mitochondrial membrane 23 homolog (yeast)
Synonyms PRO1197, TIM23, TIMM23
Calculated Molecular Weight 22 kDa
Observed Molecular Weight22 kDa
GenBank Accession NumberBC062707
Gene Symbol TIMM23
Gene ID (NCBI) 10431
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO14925
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

背景介绍

Tim23, a key component of the mitochondrial preprotein translocase, is anchored in the inner membrane by its C-terminal domain and exposes an intermediate domain in the intermembrane space that functions as a presequence receptor. Tim23 is the main component of the translocation channel of the TIM23 complex. (PMID: 10830167, PMID: 25083920)