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Tissue Factor Polyclonal antibody

Tissue Factor Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 31453-1-AP

Print datasheet

Synonyms

F3, Coagulation factor III, CD142, Thromboplastin, TFA

验证数据展示

  • WB analysis using 31453-1-AP

    WB analysis using 31453-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31453-1-AP (Coagulation Factor III/Tissue Factor antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inHepG2 cells, human placenta tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

31453-1-AP targets Tissue Factor in WB, ELISA applications and shows reactivity with human samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity human
Immunogen Recombinant protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name coagulation factor III (thromboplastin, tissue factor)
Synonyms F3, Coagulation factor III, CD142, Thromboplastin, TFA
Calculated Molecular Weight33 kDa
Observed Molecular Weight45-53 kDa
GenBank Accession NumberNM_001993.5
Gene Symbol F3
Gene ID (NCBI) 2152
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
UNIPROT IDP13726-1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Normal blood coagulation is a complex process, involving a cascade of activation of different plasma proteins, ultimately resulting in the formation of a clot, called fibrin. Tissue Factor (TF), also named Coagulation factor III or F3, is the primary initiator of the blood coagulation cascade and plays an essential role in hemostasis (PMID: 26877187; PMID: 33796108).

实验方案

Product Specific Protocols
WB protocol for Tissue Factor antibody 31453-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols