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Tissue Factor Recombinant antibody

Tissue Factor Uni-rAbTM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

240875H3

Cat no : 83887-4-RR

Print datasheet

Synonyms

F3, Coagulation factor III, CD142, TFA, TF

验证数据展示

  • WB analysis using 83887-4-RR

    WB analysis using 83887-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83887-4-RR (Coagulation Factor III/Tissue Factor antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 83887-4-RR

    Affinity and Kinetic Characterization of 83887-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83887-4-RR against Mouse Tissue Factor were performed. The affinity constant is 0.85 nM.

经过测试的应用

Positive WB detected inA431 cells, mouse brain tissue, SH-SY5Y cells, NIH/3T3 cells, rat skin tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83887-4-RR targets Tissue Factor in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen Fusion Protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name coagulation factor III
Synonyms F3, Coagulation factor III, CD142, TFA, TF
Calculated Molecular Weight33kDa
Observed Molecular Weight48-53 kDa
Gene Symbol Tissue Factor
Gene ID (NCBI) 14066
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP20352
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Normal blood coagulation is a complex process, involving a cascade of activation of different plasma proteins, ultimately resulting in the formation of a clot, called fibrin. Tissue Factor (TF), also named Coagulation factor III or F3, is the primary initiator of the blood coagulation cascade and plays an essential role in hemostasis (PMID: 26877187; PMID: 33796108). This antibody is specific for mouse Tissue Factor.

实验方案

Product Specific Protocols
WB protocol for Tissue Factor antibody 83887-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols