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Tissue Factor Polyclonal antibody

Tissue Factor Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat No : 28005-1-AP

Print datasheet

Synonyms

CD142, Coagulation factor III, F3, TF, TFA, Thromboplastin, Tissue factor

验证数据展示

  • WB analysis using 28005-1-AP

    WB analysis using 28005-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28005-1-AP (Tissue Factor antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inA431 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

28005-1-AP targets Tissue Factor in WB, ELISA applications and shows reactivity with Human samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity Human
Immunogen Tissue Factor fusion protein Ag27553 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name coagulation factor III (thromboplastin, tissue factor)
Synonyms CD142, Coagulation factor III, F3, TF, TFA, Thromboplastin, Tissue factor
Calculated Molecular Weight 295 aa, 33 kDa
Observed Molecular Weight45 kDa
GenBank Accession NumberBC011029
Gene Symbol F3
Gene ID (NCBI) 2152
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP13726
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Normal blood coagulation is a complex process, involving a cascade of activation of different plasma proteins, ultimately resulting in the formation of a clot, called fibrin. Tissue Factor (TF), also named Coagulation factor III or F3, is the primary initiator of the blood coagulation cascade and plays an essential role in hemostasis (PMID: 26877187; PMID: 33796108).

实验方案

Product Specific Protocols
WB protocol for Tissue Factor antibody 28005-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols