Tissue Factor Polyclonal antibody
Tissue Factor Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
WB, IHC, IF/ICC, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | A431 cells |
| Positive IHC detected in | human stomach cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | BxPC-3 cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
产品信息
28005-1-AP targets Tissue Factor in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.
| Tested Applications | WB, IHC, IF/ICC, ELISA Application Description |
| Cited Applications | WB |
| Tested Reactivity | human |
| Cited Reactivity | human |
| Immunogen | Tissue Factor fusion protein Ag27553 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | coagulation factor III (thromboplastin, tissue factor) |
| Synonyms | F3, Coagulation factor III, CD142, Thromboplastin, TFA |
| Calculated Molecular Weight | 295 aa, 33 kDa |
| Observed Molecular Weight | 45 kDa |
| GenBank Accession Number | BC011029 |
| Gene Symbol | F3 |
| Gene ID (NCBI) | 2152 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P13726 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Normal blood coagulation is a complex process, involving a cascade of activation of different plasma proteins, ultimately resulting in the formation of a clot, called fibrin. Tissue Factor (TF), also named Coagulation factor III or F3, is the primary initiator of the blood coagulation cascade and plays an essential role in hemostasis (PMID: 26877187; PMID: 33796108).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Tissue Factor antibody 28005-1-AP | Download protocol |
| IHC protocol for Tissue Factor antibody 28005-1-AP | Download protocol |
| IF protocol for Tissue Factor antibody 28005-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |