TNF-alpha Recombinant antibody
TNF-alpha Uni-rAbTM Recombinant Antibody for WB, IF/ICC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
mouse
Applications
WB, IF/ICC, ELISA
Conjugate
Unconjugated
CloneNo.
240702B7
验证数据展示
经过测试的应用
Positive WB detected in | PMA, LPS and Brefeldin A treated RAW 264.7 cells |
Positive IF/ICC detected in | LPS and Brefeldin A treated RAW 264.7 cells |
推荐稀释比
Application | Dilution |
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Western Blot (WB) | WB : 1:2000-1:10000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:125-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
The immunogen of 80258-6-RR is TNF-alpha Fusion Protein expressed in E. coli.
Tested Applications | WB, IF/ICC, ELISA Application Description |
Tested Reactivity | mouse |
Immunogen | Fusion Protein 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Full Name | tumor necrosis factor |
Synonyms | Tnf, Tnfa, TNF alpha,TNF-a,TNF-alpha,TNFα, TNF alpha, TNF a |
Observed Molecular Weight | 25-26 kDa |
GenBank Accession Number | NM-013693 |
Gene Symbol | TNF-alpha |
Gene ID (NCBI) | 21926 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purfication |
UNIPROT ID | P06804 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for TNF-alpha antibody 80258-6-RR | Download protocol |
IF protocol for TNF-alpha antibody 80258-6-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |