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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
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Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 66562-1-Ig (same clone as 66562-1-PBS)

    WB analysis using 66562-1-Ig (same clone as 66562-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66562-1-Ig (Tom22 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66562-1-PBS in a different storage buffer formulation.

  • IHC staining of human kidney using 66562-1-Ig (same clone as 66562-1-PBS)

    IHC staining of human kidney using 66562-1-Ig (same clone as 66562-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66562-1-Ig (Tom22 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66562-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 66562-1-Ig (same clone as 66562-1-PBS)

    IF Staining of HepG2 using 66562-1-Ig (same clone as 66562-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Tom22 antibody (66562-1-Ig, Clone: 1C2B11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-phalloidin (red). This data was developed using the same antibody clone with 66562-1-PBS in a different storage buffer formulation.

  • FC experiment of HEK-293 using 66562-1-Ig (same clone as 66562-1-PBS)

    FC experiment of HEK-293 using 66562-1-Ig (same clone as 66562-1-PBS)

    1X10^6 HEK-293 cells were intracellularly stained with 0.4 ug Anti-Human Tom22 (66562-1-Ig, Clone:1C2B11) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66562-1-PBS in a different storage buffer formulation.

Tom22 Monoclonal antibody, PBS Only

Tom22 Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA
Cat No. 66562-1-PBS

产品说明书

CloneNo. 1C2B11

宿主/亚型

Mouse / IgG2a

种属反应性

human

应用

WB, IHC, IF/ICC, FC (Intra), ELISA

别名

TOMM22, MSTP065, MST065, hTom22, 1C9 2

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

66562-1-PBS targets Tom22 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原 Tom22 fusion protein Ag1797 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 translocase of outer mitochondrial membrane 22 homolog (yeast)
别名 TOMM22, MSTP065, MST065, hTom22, 1C9 2
计算分子量 15 kDa
观测分子量 22 kDa
GenBank蛋白编号BC009363
基因名称 TOMM22/Tom22
Gene ID (NCBI) 56993
RRIDAB_2881923
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9NS69
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Tom22 is a central receptor component of the translocase of the outer membrane of mitochondria responsible for the recognition and translocation of cytosolically synthesized mitochondrial preproteins. It is showed in UniProt that phosphorylation is performed at three positions of this protein which may increase the molecular weight. Catalog#66562-1-Ig recognises  the  22 kDa  protein larger than the calculated MW which is 15 kDa. Besides, it has been reported the MV of TOMM22 is 22 kDa (PMID:19285029).