TrkB Recombinant antibody
TrkB Uni-rAbTM Recombinant Antibody for WB, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IP, ELISA
Conjugate
Unconjugated
CloneNo.
241473G5
验证数据展示
经过测试的应用
Positive WB detected in | rat brain tissue, mouse cerebellum tissue |
Positive IP detected in | mouse cerebellum tissue |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
80878-6-RR targets TrkB in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Applications | WB, IP, ELISA Application Description |
Tested Reactivity | human, mouse, rat |
Immunogen | TrkB fusion protein Ag3790 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Full Name | neurotrophic tyrosine kinase, receptor, type 2 |
Synonyms | NTRK2, NTRK 2, GP145-TrkB, GP145 TrkB, BDNF/NT-3 growth factors receptor |
Calculated Molecular Weight | 822 aa, 92 kDa |
Observed Molecular Weight | 90-95 kDa |
GenBank Accession Number | BC031835 |
Gene Symbol | TrkB |
Gene ID (NCBI) | 4915 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purfication |
UNIPROT ID | Q16620 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Tropomyosin-related kinase B (TrkB) is a member of the neurotrophic tyrosine receptor kinase (NTRK) family. It is a single-pass type 1 membrane protein consisting of three domains: an extracellular ligand binding domain, a transmembrane domain, and an intracellular tyrosine kinase domain. TrkB is primarily expressed in the central and peripheral nervous systems. TrkB plays an important role in neural development and maintenance by regulating neuronal differentiation, proliferation, survival, migration, neurogenesis, and plasticity. This antibody raised against 41-433aa of human TrkB recognizes the full-length form (140-145 kDa) and truncated form (90-95 kDa) of TrkB (PMID: 23526925; 21039416).
实验方案
Product Specific Protocols | |
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WB protocol for TrkB antibody 80878-6-RR | Download protocol |
IP protocol for TrkB antibody 80878-6-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |