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验证数据展示

  • WB analysis using 31291-1-AP

    WB analysis using 31291-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31291-1-AP (Tyrosinase antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human skin cancer using 31291-1-AP

    IHC staining of human skin cancer using 31291-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 31291-1-AP (Tyrosinase antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tyrosinase Polyclonal antibody

Tyrosinase Polyclonal Antibody for WB, IHC, ELISA
Cat No. 31291-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

TYR, OCAIA, OCA1A, Monophenol monooxygenase, LB24-AB

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inA375 cells, mouse liver tissue, mouse skin tissue, rat skin tissue
Positive IHC detected inhuman skin cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

31291-1-AP targets Tyrosinase in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 Tyrosinase fusion protein Ag34940 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 tyrosinase (oculocutaneous albinism IA)
别名 TYR, OCAIA, OCA1A, Monophenol monooxygenase, LB24-AB
计算分子量 529 aa, 60 kDa
观测分子量 70 kDa
GenBank蛋白编号BC027179
基因名称 TYR
Gene ID (NCBI) 7299
RRIDAB_3669932
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity Purification
UNIPROT IDP14679
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Tyrosinase is a copper-containing oxidase that functions in the formation of pigments such as melanins and other polyphenolic compounds. It's a melanocyte-specific enzyme critical for the synthesis of melanin, a process normally restricted to a post-Golgi compartment termed the melanosome. Loss-of-function mutations in tyrosinase are the cause of oculocutaneous albinism. The 60-kDa tyrosinase core polypeptide is modified in the endoplasmic reticulum (ER) by cotranslational addition of multiple N-linked glycans, producing the 70-kDa species. Complex sugar modifications in the Golgi apparatus further increases tyrosinase's molecular mass to 80 kDa, the size of the mature wild-type (WT) isoform. (PMID:10823941)

实验方案

Product Specific Protocols
WB protocol for Tyrosinase antibody 31291-1-APDownload protocol
IHC protocol for Tyrosinase antibody 31291-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols