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U2AF2/U2AF65 Monoclonal antibody, PBS Only

U2AF2/U2AF65 Monoclonal Antibody for WB, IF, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, rat, mouse

Applications

WB, IF, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1B7E7

Cat No : 68166-1-PBS

Print datasheet

Synonyms

hU2AF(65), hU2AF65, U2AF2, U2AF65

验证数据展示

  • IHC staining of mouse liver using 68166-1-Ig (same clone as 68166-1-PBS)

    IHC staining of mouse liver using 68166-1-Ig (same clone as 68166-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 68166-1-Ig (U2AF2 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68166-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with U2AF2 antibody included
  • IF Staining of HEK-293 using 68166-1-Ig (same clone as 68166-1-PBS)

    IF Staining of HEK-293 using 68166-1-Ig (same clone as 68166-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using U2AF2 antibody (68166-1-Ig, Clone: 1B7E7 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68166-1-PBS in a different storage buffer formulation.

  • WB analysis using 68166-1-Ig (same clone as 68166-1-PBS)

    WB analysis using 68166-1-Ig (same clone as 68166-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68166-1-Ig (U2AF2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 68166-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse liver using 68166-1-Ig (same clone as 68166-1-PBS)

    IHC staining of mouse liver using 68166-1-Ig (same clone as 68166-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 68166-1-Ig (U2AF2 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68166-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with U2AF2 antibody included
  • IHC staining of rat liver using 68166-1-Ig (same clone as 68166-1-PBS)

    IHC staining of rat liver using 68166-1-Ig (same clone as 68166-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 68166-1-Ig (U2AF2 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68166-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with U2AF2 antibody included
  • WB analysis of 4T1 using 68166-1-Ig (same clone as 68166-1-PBS)

    WB analysis of 4T1 using 68166-1-Ig (same clone as 68166-1-PBS)

    4T1 cells were subjected to SDS PAGE followed by western blot with 68166-1-Ig (U2AF2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68166-1-PBS in a different storage buffer formulation.

  • IF Staining of HEK-293 using 68166-1-Ig (same clone as 68166-1-PBS)

    IF Staining of HEK-293 using 68166-1-Ig (same clone as 68166-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using U2AF2 antibody (68166-1-Ig, Clone: 1B7E7 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68166-1-PBS in a different storage buffer formulation.

  • IF Staining of HEK-293 using 68166-1-Ig (same clone as 68166-1-PBS)

    IF Staining of HEK-293 using 68166-1-Ig (same clone as 68166-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using U2AF2 antibody (68166-1-Ig, Clone: 1B7E7 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 68166-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy U2AF2 Ready-To-Use IHC Kit. U2AF2 primary antibody included.

产品信息

68166-1-PBS targets U2AF2/U2AF65 in WB, IF, IHC, Indirect ELISA applications and shows reactivity with Human, rat, mouse samples.

Tested Applications WB, IF, IHC, Indirect ELISA Application Description
Tested Reactivity Human, rat, mouse
Immunogen U2AF2/U2AF65 fusion protein Ag8070 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name U2 small nuclear RNA auxiliary factor 2
Synonyms hU2AF(65), hU2AF65, U2AF2, U2AF65
Calculated Molecular Weight 54 kDa
Observed Molecular Weight60-65 kDa
GenBank Accession NumberBC008740
Gene Symbol U2AF2
Gene ID (NCBI) 11338
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP26368
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

背景介绍

U2AF2, also named as U2 auxiliary factor 65 kDa subunit, is a 475 amino acid protein, which belongs to the splicing factor SR family. U2AF2 is Necessary for the splicing of pre-mRNA. By recruiting PRPF19 and the PRP19C/Prp19 complex/NTC/Nineteen complex to the RNA polymerase II C-terminal domain (CTD), pre-mRNA may couple transcription to splicing. The calculated molecular weight of U2AF2 is 53 kDa, but modified U2AF2 is about 65 kDa.