验证数据展示
经过测试的应用
| Positive WB detected in | COLO 320 cells, HeLa cells |
| Positive IHC detected in | mouse small intestine tissue, human prostate cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 1 publications below |
| WB | See 10 publications below |
产品信息
10334-1-AP targets U2AF35 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Applications | WB, IHC, ELISA Application Description |
| Cited Applications | WB |
| Tested Reactivity | human, mouse |
| Cited Reactivity | human |
| Immunogen | U2AF35 fusion protein Ag0399 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | U2 small nuclear RNA auxiliary factor 1 |
| Synonyms | U2AF1, U2AFBP, RNU2AF1, RN, FP793 |
| Calculated Molecular Weight | 28 kDa |
| Observed Molecular Weight | 35 kDa |
| GenBank Accession Number | BC001923 |
| Gene Symbol | U2AF1 |
| Gene ID (NCBI) | 7307 |
| RRID | AB_2211314 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q01081 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
U2 auxiliary factor (U2AF), comprising a large and a small subunit, is a non-snRNP protein required for the binding of U2 snRNP to the pre-mRNA branch site. U2 (RNU2) small nuclear RNA auxiliary factor 1 (U2AF1,synonyms: RN, FP793, U2AF35, U2AFBP, RNU2AF1)is the small subunit which plays a critical role in both constitutive and enhancer-dependent RNA splicing by directly mediating interactions between the large subunit and proteins bound to the enhancers. The U2AF1 gene is localized to chromosome 21q22.3, which is the critical region for three diseases, progressive myoclonus epilepsy, autoimmune polyglandular disease type 1, and one form of bipolar affective disorder.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for U2AF35 antibody 10334-1-AP | Download protocol |
| IHC protocol for U2AF35 antibody 10334-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Mol Cell U2AF35(S34F) Promotes Transformation by Directing Aberrant ATG7 Pre-mRNA 3' End Formation. | ||
Nucleic Acids Res PUF60-activated exons uncover altered 3' splice-site selection by germline missense mutations in a single RRM. | ||
Nucleic Acids Res Identification of U2AF(35)-dependent exons by RNA-Seq reveals a link between 3' splice-site organization and activity of U2AF-related proteins. | ||
Int J Mol Sci Splicing Enhancers at Intron-Exon Borders Participate in Acceptor Splice Sites Recognition. | ||
J Biol Chem Splicing factor SPF30 bridges an interaction between the prespliceosome protein U2AF35 and tri-small nuclear ribonucleoprotein protein hPrp3. |
