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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 60289-1-Ig (same clone as 60289-1-PBS)

    WB analysis using 60289-1-Ig (same clone as 60289-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 60289-1-Ig (U2AF35 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60289-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 60289-1-Ig (same clone as 60289-1-PBS)

    WB analysis of HeLa using 60289-1-Ig (same clone as 60289-1-PBS)

    WB result of U2AF35 antibody (60289-1-Ig; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-U2AF35 transfected HeLa cells. This data was developed using the same antibody clone with 60289-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 60289-1-Ig (same clone as 60289-1-PBS)

    IHC staining of human breast cancer using 60289-1-Ig (same clone as 60289-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60289-1-Ig (U2AF35 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60289-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with U2AF1 antibody included
  • IP experiment of HeLa using 60289-1-Ig (same clone as 60289-1-PBS)

    IP experiment of HeLa using 60289-1-Ig (same clone as 60289-1-PBS)

    IP result of anti-U2AF35 (IP:60289-1-Ig, 3ug; Detection:60289-1-Ig 1:500) with HeLa cells lysate 3000ug. This data was developed using the same antibody clone with 60289-1-PBS in a different storage buffer formulation.

  • IF Staining of HEK-293 using 60289-1-Ig (same clone as 60289-1-PBS)

    IF Staining of HEK-293 using 60289-1-Ig (same clone as 60289-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using U2AF35 antibody (60289-1-Ig, Clone: 2B7F11 ) at dilution of 1:2000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 60289-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 60289-1-Ig (same clone as 60289-1-PBS)

    IF Staining of HepG2 using 60289-1-Ig (same clone as 60289-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using U2AF35 antibody (60289-1-Ig, Clone: 2B7F11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60289-1-PBS in a different storage buffer formulation.

  • FC experiment of Ramos using 60289-1-Ig (same clone as 60289-1-PBS)

    FC experiment of Ramos using 60289-1-Ig (same clone as 60289-1-PBS)

    1X10^6 Ramos cells were intracellularly stained with 0.4 ug Anti-Human U2AF35 (60289-1-Ig, Clone:2B7F11) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 60289-1-PBS in a different storage buffer formulation.

U2AF35 Monoclonal antibody, PBS Only

U2AF35 Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA
Cat No. 60289-1-PBS

产品说明书

CloneNo. 2B7F11

宿主/亚型

Mouse / IgG2c

种属反应性

rat, mouse, human

应用

WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

别名

U2AF1, U2AFBP, RNU2AF1, RN, FP793

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy U2AF1 Ready-To-Use IHC Kit. U2AF1 primary antibody included.

产品信息

60289-1-PBS targets U2AF35 in WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA applications and shows reactivity with rat, mouse, human samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA Application Description
经测试反应性 rat, mouse, human
免疫原 U2AF35 fusion protein Ag0399 种属同源性预测
宿主/亚型 Mouse / IgG2c
抗体类别 Monoclonal
产品类型 Antibody
全称 U2 small nuclear RNA auxiliary factor 1
别名 U2AF1, U2AFBP, RNU2AF1, RN, FP793
计算分子量 28 kDa
观测分子量 35-40 kDa
GenBank蛋白编号BC001923
基因名称 U2AF1
Gene ID (NCBI) 7307
RRIDAB_2858277
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ01081
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

U2 auxiliary factor (U2AF), comprising a large and a small subunit, is a non-snRNP protein required for the binding of U2 snRNP to the pre-mRNA branch site. U2 (RNU2) small nuclear RNA auxiliary factor 1 (U2AF1,synonyms: RN, FP793, U2AF35, U2AFBP, RNU2AF1)is the small subunit which plays a critical role in both constitutive and enhancer-dependent RNA splicing by directly mediating interactions between the large subunit and proteins bound to the enhancers. The U2AF1 gene is localized to chromosome 21q22.3, which is the critical region for three diseases, progressive myoclonus epilepsy, autoimmune polyglandular disease type 1, and one form of bipolar affective disorder. The calculated molecular weight of U2AF35 is 28 kDa, but the modified U2AF35 protein is about 35-40 kDa.