验证数据展示
经过测试的应用
| Positive WB detected in | A375 cells, HEK-293 cells, Neuro-2a cells, Y79 cells, HeLa cells |
| Positive IHC detected in | human colon tissue, human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | A375 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:12000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
25654-1-AP targets UACA in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, canine |
| 免疫原 | UACA fusion protein Ag22438 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | uveal autoantigen with coiled-coil domains and ankyrin repeats |
| 别名 | KIAA1561, NUCLING, Uveal autoantigen with coiled-coil domains and ankyrin repeats |
| 计算分子量 | 1416 aa, 163 kDa |
| 观测分子量 | 163 kDa |
| GenBank蛋白编号 | BC113407 |
| 基因名称 | UACA |
| Gene ID (NCBI) | 55075 |
| RRID | AB_2880179 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9BZF9 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for UACA antibody 25654-1-AP | Download protocol |
| IHC protocol for UACA antibody 25654-1-AP | Download protocol |
| IF protocol for UACA antibody 25654-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Aging (Albany NY) Transcriptome and proteome analysis of the antitumor activity of maslinic acid against pancreatic cancer cells | ||
J Oncol Maslinic Acid Inhibits the Growth of Malignant Gliomas by Inducing Apoptosis via MAPK Signaling. | ||
Cell Rep Rab39 and its effector UACA regulate basolateral exosome release from polarized epithelial cells. | ||
Int J Clin Exp Pathol Knockdown of UACA inhibitsproliferation and invasion and promotes senescence of hepatocellular carcinoma cells
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