验证数据展示
经过测试的应用
| Positive WB detected in | DU 145 cells, PC-3 cells |
| Positive IF/ICC detected in | PC-3 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
产品信息
16318-1-AP targets UAP1 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human |
| 文献引用反应性 | mouse |
| 免疫原 | UAP1 fusion protein Ag9371 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | UDP-N-acteylglucosamine pyrophosphorylase 1 |
| 别名 | EC:2.7.4.-, AntigenX, Antigen X, AGX1, AGX 2 |
| 计算分子量 | 505 aa, 57 kDa |
| 观测分子量 | 57-59 kDa |
| GenBank蛋白编号 | BC009377 |
| 基因名称 | UAP1 |
| Gene ID (NCBI) | 6675 |
| RRID | AB_2878242 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q16222 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
UAP1 can convert UTP and GlcNAc-1-P into UDP-GlcNAc, it can also convert UTP and GalNAc-1-P into UDP-GalNAc. There are three isoforms of UAP1, among which isoform AGX1 has 2 to 3 times higher activity towards GalNAc-1-P, while isoform AGX2 has 8 times more activity towards GlcNAc-1-P. It has been reported that UAP1 is specific and highly expressed in prostate cancer. UAP1 can protect cells against inhibitors of N-linked glycosylation. UAP1 may play an important role in maintaining INS responsiveness.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for UAP1 antibody 16318-1-AP | Download protocol |
| IF protocol for UAP1 antibody 16318-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |