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验证数据展示

  • WB analysis using 67545-1-Ig (same clone as 67545-1-PBS)

    WB analysis using 67545-1-Ig (same clone as 67545-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67545-1-Ig (UAP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67545-1-PBS in a different storage buffer formulation.

  • IHC staining of human pancreas cancer using 67545-1-Ig (same clone as 67545-1-PBS)

    IHC staining of human pancreas cancer using 67545-1-Ig (same clone as 67545-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 67545-1-Ig (UAP1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67545-1-PBS in a different storage buffer formulation.

  • IF Staining of PC-3 using 67545-1-Ig (same clone as 67545-1-PBS)

    IF Staining of PC-3 using 67545-1-Ig (same clone as 67545-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed PC-3 cells using UAP1 antibody (67545-1-Ig, Clone: 2C7B2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67545-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

UAP1 Monoclonal antibody, PBS Only

UAP1 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 67545-1-PBS

产品说明书

CloneNo. 2C7B2

宿主/亚型

Mouse / IgG1

种属反应性

Human

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

AgX, AGX 2, AGX1, Antigen X, SPAG2, UAP1

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

67545-1-PBS targets UAP1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 Human
免疫原 UAP1 fusion protein Ag9513 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 UDP-N-acteylglucosamine pyrophosphorylase 1
别名 AgX, AGX 2, AGX1, Antigen X, SPAG2, UAP1
计算分子量 505 aa, 57 kDa
观测分子量 57-59 kDa
GenBank蛋白编号BC009377
基因名称 UAP1
Gene ID (NCBI) 6675
RRIDAB_2882762
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ16222
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

UAP1 can convert UTP and GlcNAc-1-P into UDP-GlcNAc, it can also convert UTP and GalNAc-1-P into UDP-GalNAc. There are three isoforms of UAP1, among which isoform  AGX1  has 2 to 3 times higher activity towards GalNAc-1-P, while isoform  AGX2  has 8 times more activity towards GlcNAc-1-P. It has been reported that UAP1 is specific and highly expressed in prostate cancer. UAP1 can protect cells against inhibitors of N-linked glycosylation. UAP1 may play an important role in maintaining INS responsiveness. 67545-1-Ig detects 57 and 59 kDa isoforms in SDS-PAGE.(PMID:25241896)