验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, HeLa cells, Jurkat cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells, HeLa cells |
| Positive FC (Intra) detected in | HeLa cells |
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
12093-1-AP targets UBA5 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, IF/ICC, FC (Intra), IP, ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, mouse |
| 免疫原 | UBA5 fusion protein Ag2734 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | ubiquitin-like modifier activating enzyme 5 |
| 别名 | Ubiquitin-like modifier-activating enzyme 5, Ubiquitin-activating enzyme E1 domain-containing protein 1, Ubiquitin-activating enzyme 5, Ubiquitin activating enzyme 5, UBE1DC1 |
| 计算分子量 | 404 aa, 45 kDa |
| 观测分子量 | 45 kDa |
| GenBank蛋白编号 | BC009737 |
| 基因名称 | UBA5 |
| Gene ID (NCBI) | 79876 |
| RRID | AB_2211743 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9GZZ9 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
UBA5(Ubiquitin-like modifier-activating enzyme 5) is also named as UBE1DC1,ThiFP1.It has been detected highest UBE1DC1 expression in spleen, high expression in most other tissues examined, and low expression in colon, prostate, brain, and ovary by RT-PCR analysis(PMID:16328888).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for UBA5 antibody 12093-1-AP | Download protocol |
| IHC protocol for UBA5 antibody 12093-1-AP | Download protocol |
| IF protocol for UBA5 antibody 12093-1-AP | Download protocol |
| IP protocol for UBA5 antibody 12093-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Mol Cell UFL1 ablation in T cells suppresses PD-1 UFMylation to enhance anti-tumor immunity
| ||
Am J Hum Genet Biallelic Variants in UBA5 Reveal that Disruption of the UFM1 Cascade Can Result in Early-Onset Encephalopathy. | ||
J Cell Sci ACSL3 is a novel GABARAPL2 interactor that links ufmylation and lipid droplet biogenesis. | ||
BMC Cardiovasc Disord Screening UFMylation-associated genes in heart tissues of Ufm1-transgenic mice |
