验证数据展示
经过测试的应用
| Positive WB detected in | mouse bone marrow |
| Positive IP detected in | mouse spleen tissue |
| Positive IF/ICC detected in | THP-1 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 7 publications below |
产品信息
15823-1-AP targets UBASH3A-Specific in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 | Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | ubiquitin associated and SH3 domain containing, A |
| 别名 | Cbl interacting protein 4, CLIP4, STS 2, STS2, T cell ubiquitin ligand, TULA, UBASH3A, UBASH3A-Specific |
| 计算分子量 | 74 kDa |
| 观测分子量 | 70-74 kDa |
| GenBank蛋白编号 | NM_018961 |
| 基因名称 | UBASH3A |
| Gene ID (NCBI) | 53347 |
| RRID | AB_2272584 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P57075 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
UBASH3A gene encodes ubiquitin-associated and SH3 domain-containing protein A (UBASH3A) also known as T-cell ubiquitin ligand 1 (TULA1) which is one of two family members belonging to the T-cell ubiquitin ligand (TULA) family. UBASH3A gene expression is limited to only a few tissues, with its highest expression in spleen, peripheral blood leukocytes, and bone marrow. UBASH3A protein is associated with c-Cbl and ubiquitylated proteins and has been implicated in the regulation of signaling mediated by protein-tyrosine kinases oth family members can negatively regulate T-cell signaling. UBASH3A can facilitate growth factor withdrawal-induced apoptosis in T cells via its interaction with apoptosis-inducing factor (AIF). This antibody is UBASH3A-specific.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for UBASH3A-Specific antibody 15823-1-AP | Download protocol |
| IF protocol for UBASH3A-Specific antibody 15823-1-AP | Download protocol |
| IP protocol for UBASH3A-Specific antibody 15823-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Diabetes UBASH3A Mediates Risk for Type 1 Diabetes Through Inhibition of T-Cell Receptor-Induced NF-κB Signaling. | ||
Int J Mol Sci UBASH3A Interacts with PTPN22 to Regulate IL2 Expression and Risk for Type 1 Diabetes | ||
BMC Cancer FLI1 induces erythroleukemia through opposing effects on UBASH3A and UBASH3B expression | ||
Immunity TCR signaling promotes formation of an STS1-Cbl-b complex with pH-sensitive phosphatase activity that suppresses T cell function in acidic environments | ||