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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 67482-1-Ig (same clone as 67482-1-PBS)

    WB analysis using 67482-1-Ig (same clone as 67482-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67482-1-Ig (UBC12 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67482-1-PBS in a different storage buffer formulation.

  • WB analysis of HEK-293 using 67482-1-Ig (same clone as 67482-1-PBS)

    WB analysis of HEK-293 using 67482-1-Ig (same clone as 67482-1-PBS)

    WB result of UBC12 antibody (67482-1-Ig; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-UBC12 transfected HEK-293 cells. This data was developed using the same antibody clone with 67482-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 67482-1-Ig (same clone as 67482-1-PBS)

    IHC staining of human liver cancer using 67482-1-Ig (same clone as 67482-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67482-1-Ig (UBC12 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67482-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 67482-1-Ig (same clone as 67482-1-PBS)

    IHC staining of human liver cancer using 67482-1-Ig (same clone as 67482-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67482-1-Ig (UBC12 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67482-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 67482-1-Ig (same clone as 67482-1-PBS)

    IF Staining of HeLa using 67482-1-Ig (same clone as 67482-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using UBC12 antibody (67482-1-Ig, Clone: 1H8D2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 67482-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

UBC12 Monoclonal antibody, PBS Only

UBC12 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 67482-1-PBS

产品说明书

CloneNo. 1H8D2

宿主/亚型

Mouse / IgG1

种属反应性

Human, Mouse, Rat

应用

WB, IHC, IF/ICC, ELISA

别名

hUbc12, NEDD8 carrier protein, NEDD8 conjugating enzyme Ubc12, NEDD8 protein ligase, UBC RS2, UBC12, UBE2M

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

67482-1-PBS targets UBC12 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 Human, Mouse, Rat
免疫原 UBC12 fusion protein Ag5984 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 ubiquitin-conjugating enzyme E2M (UBC12 homolog, yeast)
别名 hUbc12, NEDD8 carrier protein, NEDD8 conjugating enzyme Ubc12, NEDD8 protein ligase, UBC RS2, UBC12, UBE2M
计算分子量 21 kDa
观测分子量 21 kDa
GenBank蛋白编号BC058924
基因名称 UBC12
Gene ID (NCBI) 9040
RRIDAB_2882709
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP61081
储存缓冲液 PBS Only
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C