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Host
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Species Reactivity
0
Species Reactivity
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Conjugate
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Compatible Species
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Conjugate
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验证数据展示

  • WB analysis of mouse spleen using 10224-1-AP (same clone as 10224-1-PBS)

    WB analysis of mouse spleen using 10224-1-AP (same clone as 10224-1-PBS)

    mouse spleen tissue were subjected to SDS PAGE followed by western blot with 10224-1-AP (UBC9 antibody) at dilution of 1:50 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10224-1-PBS in a different storage buffer formulation.

  • IHC staining of human lung cancer using 10224-1-AP (same clone as 10224-1-PBS)

    IHC staining of human lung cancer using 10224-1-AP (same clone as 10224-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer using 10224-1-AP (UBC9 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 10224-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with UBE2I/UBC9 antibody included
  • IHC staining of human lung cancer using 10224-1-AP (same clone as 10224-1-PBS)

    IHC staining of human lung cancer using 10224-1-AP (same clone as 10224-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer using 10224-1-AP (UBC9 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 10224-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with UBE2I/UBC9 antibody included

UBC9 Polyclonal antibody, PBS Only

UBC9 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 10224-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, Indirect ELISA

别名

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy UBE2I/UBC9 Ready-To-Use IHC Kit. UBE2I/UBC9 primary antibody included.

产品信息

10224-1-PBS targets UBC9 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 UBC9 fusion protein Ag0252 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ubiquitin-conjugating enzyme E2I (UBC9 homolog, yeast)
别名
计算分子量 18 kDa
观测分子量 21 kDa
GenBank蛋白编号BC000427
基因名称 UBC9
Gene ID (NCBI) 7329
RRIDAB_2210593
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP63279
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

UBC9 is also named as UBE2I, UBCE9 and belongs to the ubiquitin-conjugating enzyme family. It is a homologue of the E2 ubiquitin conjugating enzyme and participates in the covalent linking of SUMO-1 molecule to the target protein. This protein is present at a high level in spleen and lung. Moderate level of UBC9 is detected in kidney and liver. Low amount of UBC9 is observed in brain, whereas the 18 kDa band of UBC9 is barely visible or absent in heart and skeletal muscle. In heart and muscle extracts the UBC9 antibodies recognizes a 38 kDa protein band,but this band is not visible in extracts of other rat tissues(PMID:14739995).