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FAT10 Polyclonal antibody

FAT10 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 13003-2-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human and More (2)

应用

WB, IHC, ELISA and More (1)

Diubiquitin, FAT10, GABBR1, UBD, UBD 3, ubiquitin D, Ubiquitin like protein FAT10

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inTNF alpha and IFN gamma treated HepG2 cells
Positive IHC detected inhuman tonsillitis tissue, human lung cancer tissue, human lymphoma tissue, human stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy UBD Ready-To-Use IHC Kit. UBD primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13003-2-AP targets FAT10 in WB, IHC, IF, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB, IHC, IF
经测试反应性 human
文献引用反应性human, mouse, rat
免疫原 FAT10 fusion protein Ag3680 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ubiquitin D
别名 Diubiquitin, FAT10, GABBR1, UBD, UBD 3, ubiquitin D, Ubiquitin like protein FAT10
计算分子量 165 aa, 18 kDa
观测分子量20 kDa, 70kDa
GenBank蛋白编号BC012472
基因名称 FAT10
Gene ID (NCBI) 10537
RRIDAB_2304122
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO15205
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

FAT10, also named UBD, contains two ubiquitin-like domains. It is a ubiquitin-like protein modifier that can be covalently attached to the target protein and subsequently leads to their degradation by the 26S proteasome, in a NUB1L-dependent manner. FAT10 also has important roles in cell mitosis, chromosome instability, apoptosis, and immune response. FAT10 mediates apoptosis in a caspase-dependent manner, especially in the renal epithelium and tubular cells during renal diseases such as polycystic kidney disease and Human immunodeficiency virus (HIV)-associated nephropathy (HIVAN). It promotes the expression of the proteasome subunit beta type-9 (PSMB9/LMP2). FAT10 regulates TNF-alpha-induced and LPS-mediated activation of the central mediator of innate immunity NF-kappa-B by promoting TNF-alpha-mediated proteasomal degradation of ubiquitinated-I-kappa-B-alpha. It may be involved in dendritic cell (DC) maturation, the process by which immature dendritic cells differentiate into fully competent antigen-presenting cells that initiate T-cell responses. FAT10 may be a marker for precancerous lesions and may promote cancer progression. This antibody is a rabbit polyclonal antibody raised against full-length FAT10 of human origin.

实验方案

Product Specific Protocols
WB protocol for FAT10 antibody 13003-2-APDownload protocol
IHC protocol for FAT10 antibody 13003-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IHC,IF

Cancer Res

The Ubiquitin-like Protein FAT10 Stabilizes eEF1A1 Expression to Promote Tumor Proliferation in a Complex Manner.

Authors - Xiuxia Liu
  • KD Validated
mouseWB,IF

J Biol Chem

Ubiquitin D regulates IRE1α/JNK-dependent apoptosis in pancreatic beta cells.

Authors - Flora Brozzi
humanWB

Environ Toxicol

Increased UBD Is a Potential Diagnostic and Prognostic Biomarker in Glioma

Authors - Tao Wu
mouseIHC

DNA Res

Identification of novel genes selectively expressed in the follicle-associated epithelium from the meta-analysis of transcriptomics data from multiple mouse cell and tissue populations.

Authors - Kobayashi Atsushi A
ratIHC

Toxicol Lett

Ochratoxin A induces karyomegaly and cell cycle aberrations in renal tubular cells without relation to induction of oxidative stress responses in rats.

Authors - Taniai Eriko E
mouseIF

Immunobiology

c-Rel is dispensable for the differentiation and functional maturation of M cells in the follicle-associated epithelium.

Authors - Anuj Sehgal
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