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UBE1 Polyclonal antibody

UBE1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 15912-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

UBA1, GXP1, EC:6.2.1.45, AMCX1, A1ST

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inHL-60 cells, HeLa cells, HEK-293 cells, mouse spleen tissue, rat spleen tissue, mouse colon tissue
Positive IHC detected inhuman colon cancer tissue, human normal colon
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy UBA1 Ready-To-Use IHC Kit. UBA1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:200-1:1500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15912-1-AP targets UBE1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB, IHC
经测试反应性 human, mouse, rat
文献引用反应性human, mouse
免疫原 UBE1 fusion protein Ag8703 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ubiquitin-like modifier activating enzyme 1
别名 UBA1, GXP1, EC:6.2.1.45, AMCX1, A1ST
计算分子量 1058 aa, 118 kDa
观测分子量 114-118 kDa
GenBank蛋白编号BC013041
基因名称 UBA1
Gene ID (NCBI) 7317
RRIDAB_2211462
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP22314
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

UBE1(Ubiquitin-activating enzyme E1) is also named as A1S9T, UBE1 and belongs to the ubiquitin-activating E1 family. It catalyzes the first step in ubiquitin conjugation to mark cellular proteins for degradation and initiates the activation and conjugation of ubiquitin-like proteins. Defects in UBE1 are the cause of spinal muscular atrophy X-linked type 2 (SMAX2). UBE1 has two isoforms with the molecular mass of 118 and 114 kDa.

实验方案

Product Specific Protocols
WB protocol for UBE1 antibody 15912-1-APDownload protocol
IHC protocol for UBE1 antibody 15912-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC

Cancer Discov

The UBA1-STUB1 axis mediates cancer immune escape and resistance to checkpoint blockade

Authors - Yi Bao
humanWB

Nat Commun

DNA replication initiation factor RECQ4 possesses a role in antagonizing DNA replication initiation

Authors - Xiaohua Xu
mouseIHC

ACS Nano

Graphene Oxide Causes Disordered Zonation Due to Differential Intralobular Localization in the Liver.

Authors - Yakun Wu
human

Cancer Biol Med

CSN6 promotes tumorigenesis of gastric cancer by ubiquitin-independent proteasomal degradation of p16INK4a.

Authors - Wenqi Du
humanWB

Am J Cancer Res

The E3 ubiquitin ligase RBCK1 promotes the invasion and metastasis of hepatocellular carcinoma by destroying the PPARγ/PGC1α complex.

Authors - Zheng Xu
humanIHC

Front Oncol

Ubiquitin-Like Modifier Activating Enzyme 1 as a Novel Diagnostic and Prognostic Indicator That Correlates With Ferroptosis and the Malignant Phenotypes of Liver Cancer Cells.

Authors - Yiru Shan
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