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UBE2C Polyclonal antibody

UBE2C Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA and More (2)

Conjugate

Unconjugated

Cat no : 12134-2-AP

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Synonyms

UBCH10, UBE2C, Ubiquitin carrier protein C, Ubiquitin protein ligase C



经过测试的应用

Positive WB detected inHeLa cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy UBE2C Ready-To-Use IHC Kit. UBE2C primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12134-2-AP targets UBE2C in WB, IHC, IP, CoIP, ELISA, IF applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IP, IHC, ELISA Application Description
Cited ApplicationsWB, IHC, CoIP, IF
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Immunogen UBE2C fusion protein Ag2780 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ubiquitin-conjugating enzyme E2C
Synonyms UBCH10, UBE2C, Ubiquitin carrier protein C, Ubiquitin protein ligase C
Calculated Molecular Weight 179 aa, 20 kDa
Observed Molecular Weight 20 kDa
GenBank Accession NumberBC016292
Gene Symbol UBE2C
Gene ID (NCBI) 11065
RRIDAB_2212191
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO00762
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

UBE2C(Ubiquitin-conjugating enzyme E2 C) is also known as UBCH10 and belongs to the ubiquitin-conjugating enzyme family. It is highly expressed in a variety of human primary tumors, and it is able to promote cell proliferation and malignant transformation (PMID:18703417). UBE2C, along with the E3 ligase of the anaphasepromoting complex (APC), catalyzes the ubiquitination of mitotic cyclins A and B, as well as securin. UBE2C is essential for cell cycle progression, and mutation of its active site cysteine confers a dominant-negative phenotype(PMID:17217624).

实验方案

Product Specific Protocols
WB protocol for UBE2C antibody 12134-2-APDownload protocol
IHC protocol for UBE2C antibody 12134-2-APDownload protocol
IP protocol for UBE2C antibody 12134-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
WB

Plant Cell

SINAT E3 Ubiquitin Ligases Mediate FREE1 and VPS23A Degradation to Modulate Abscisic Acid Signaling.

Authors - Fan-Nv Xia
humanWB,IF

Arterioscler Thromb Vasc Biol

Disturbed Flow Increases UBE2C (Ubiquitin E2 Ligase C) via Loss of miR-483-3p, Inducing Aortic Valve Calcification by the pVHL (von Hippel-Lindau Protein) and HIF-1α (Hypoxia-Inducible Factor-1α) Pathway in Endothelial Cells.

Authors - Joan Fernandez Esmerats
humanWB,IHC

Cell Biosci

DNA primase subunit 1 deteriorated progression of hepatocellular carcinoma by activating AKT/mTOR signaling and UBE2C-mediated P53 ubiquitination.

Authors - Mengqi Zhu
mouseIHC

J Biol Chem

The Transcription factor NF-YA is Crucial for Neural Progenitor Maintenance during Brain Development

Authors - Tomoyuki Yamanaka
humanWB

Biomed Pharmacother

Affinity-based protein profiling to reveal targets of puerarin involved in its protective effect on cardiomyocytes.

Authors - Shuai Huang
mouseWB,IHC

Front Genet

Identifying the Key Genes in Mouse Liver Regeneration After Partial Hepatectomy by Bioinformatics Analysis and in vitro/vivo Experiments.

Authors - Jian Zhao