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UBE2C Polyclonal antibody
UBE2C Polyclonal Antibody for WB, IP, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IP, IHC, ELISA and More (2)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | HeLa cells |
Positive IP detected in | HeLa cells |
Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:200-1:1000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
12134-2-AP targets UBE2C in WB, IHC, IP, CoIP, ELISA, IF applications and shows reactivity with human, mouse, rat samples.
Tested Applications | WB, IP, IHC, ELISA Application Description |
Cited Applications | WB, IHC, CoIP, IF |
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse |
Immunogen | UBE2C fusion protein Ag2780 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | ubiquitin-conjugating enzyme E2C |
Synonyms | UBCH10, UBE2C, Ubiquitin carrier protein C, Ubiquitin protein ligase C |
Calculated Molecular Weight | 179 aa, 20 kDa |
Observed Molecular Weight | 20 kDa |
GenBank Accession Number | BC016292 |
Gene Symbol | UBE2C |
Gene ID (NCBI) | 11065 |
RRID | AB_2212191 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | O00762 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for UBE2C antibody 12134-2-AP | Download protocol |
IHC protocol for UBE2C antibody 12134-2-AP | Download protocol |
IP protocol for UBE2C antibody 12134-2-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Plant Cell SINAT E3 Ubiquitin Ligases Mediate FREE1 and VPS23A Degradation to Modulate Abscisic Acid Signaling. | ||
Arterioscler Thromb Vasc Biol Disturbed Flow Increases UBE2C (Ubiquitin E2 Ligase C) via Loss of miR-483-3p, Inducing Aortic Valve Calcification by the pVHL (von Hippel-Lindau Protein) and HIF-1α (Hypoxia-Inducible Factor-1α) Pathway in Endothelial Cells. | ||
Cell Biosci DNA primase subunit 1 deteriorated progression of hepatocellular carcinoma by activating AKT/mTOR signaling and UBE2C-mediated P53 ubiquitination. | ||
J Biol Chem The Transcription factor NF-YA is Crucial for Neural Progenitor Maintenance during Brain Development | ||
Biomed Pharmacother Affinity-based protein profiling to reveal targets of puerarin involved in its protective effect on cardiomyocytes. | ||
Front Genet Identifying the Key Genes in Mouse Liver Regeneration After Partial Hepatectomy by Bioinformatics Analysis and in vitro/vivo Experiments. |