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UBE2D1/2/3/4 Polyclonal antibody, PBS Only

UBE2D1/2/3/4 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 11373-1-PBS

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Synonyms

验证数据展示

  • WB analysis using 11373-1-AP (same clone as 11373-1-PBS)

    WB analysis using 11373-1-AP (same clone as 11373-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 11373-1-AP (UBE2D1/2/3/4 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11373-1-PBS in a different storage buffer formulation.

  • WB analysis of NIH/3T3 using 11373-1-AP (same clone as 11373-1-PBS)

    WB analysis of NIH/3T3 using 11373-1-AP (same clone as 11373-1-PBS)

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 11373-1-AP (UBE2D1-Specific antibody) at dilution of 1:500. . This data was developed using the same antibody clone with 11373-1-PBS in a different storage buffer formulation.

  • IP experiment of Jurkat using 11373-1-AP (same clone as 11373-1-PBS)

    IP experiment of Jurkat using 11373-1-AP (same clone as 11373-1-PBS)

    IP result of anti-UBE2D1/2/3/4 (IP:11373-1-AP, 4ug; Detection:11373-1-AP 1:300) with Jurkat cells lysate 2600 ug. This data was developed using the same antibody clone with 11373-1-PBS in a different storage buffer formulation.

  • IHC staining of human prostate cancer using 11373-1-AP (same clone as 11373-1-PBS)

    IHC staining of human prostate cancer using 11373-1-AP (same clone as 11373-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer using 11373-1-AP (UBE2D1-Specific antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 11373-1-PBS in a different storage buffer formulation.

产品信息

11373-1-PBS targets UBE2D1/2/3/4 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IP, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen UBE2D1/2/3/4 fusion protein Ag1932 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ubiquitin-conjugating enzyme E2D 1 (UBC4/5 homolog, yeast)
Synonyms
Calculated Molecular Weight 17 kDa
Observed Molecular Weight 17 kDa
GenBank Accession NumberBC015997
Gene Symbol UBE2D1
Gene ID (NCBI) 7321
RRIDAB_2210129
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP51668
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Ubiquitination is a post-translational modification pathway or is part of the specific protein-degradation pathway by the 26S proteasome. Ubiquitination of a target protein involves multistep enzymatic reaction catalyzed by a cascade of enzymes including Ub-activating enzymes (E1s), Ub-conjugating enzymes (E2s) and Ub ligases (E3s) (PMID: 23542885). UBE2D family, which has 4 members named as UBE2D1/2/3/4, is an E2 ubiquitin-conjugating enzyme family in the ubiquitin-proteasome system. This antibody can recognize all the four members of UBE2D due to the high homology.