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验证数据展示

  • WB analysis of Jurkat using 10722-1-AP

    WB analysis of Jurkat using 10722-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 10722-1-AP (UBE2G2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human prostate cancer using 10722-1-AP

    IHC staining of human prostate cancer using 10722-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 10722-1-AP (UBE2G2 antibody) at dilution of 1:100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human prostate cancer using 10722-1-AP

    IHC staining of human prostate cancer using 10722-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 10722-1-AP (UBE2G2 antibody) at dilution of 1:100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

UBE2G2 Polyclonal antibody

UBE2G2 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 10722-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

UBC7, UBE2G2, Ubiquitin carrier protein G2, Ubiquitin protein ligase G2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inJurkat cells, HeLa cells, K-562 cells, mouse testis tissue
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:100-1:600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10722-1-AP targets UBE2G2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 UBE2G2 fusion protein Ag1082 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ubiquitin-conjugating enzyme E2G 2 (UBC7 homolog, yeast)
别名 UBC7, UBE2G2, Ubiquitin carrier protein G2, Ubiquitin protein ligase G2
计算分子量 19 kDa
观测分子量 16-19 kDa
GenBank蛋白编号BC008351
基因名称 UBE2G2
Gene ID (NCBI) 7327
RRIDAB_2256726
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP60604
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for UBE2G2 antibody 10722-1-APDownload protocol
IHC protocol for UBE2G2 antibody 10722-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols