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UBE2Q2 Polyclonal antibody

UBE2Q2 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 12581-1-AP

Print datasheet

Synonyms

UBCi, UBE2Q2, Ubiquitin carrier protein Q2, Ubiquitin protein ligase Q2

验证数据展示

  • WB analysis of A431 using 12581-1-AP

    WB analysis of A431 using 12581-1-AP

    A431 cells were subjected to SDS PAGE followed by western blot with 12581-1-AP (UBE2Q2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • IHC staining of human skin cancer using 12581-1-AP

    IHC staining of human skin cancer using 12581-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin cancer using 12581-1-AP (UBE2Q2 antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human skin cancer using 12581-1-AP

    IHC staining of human skin cancer using 12581-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin cancer using 12581-1-AP (UBE2Q2 antibody) at dilution of 1:100 (under 40x lens).

  • WB analysis of HEK-293T using 12581-1-AP

    WB analysis of HEK-293T using 12581-1-AP

    HEK-293T cells were subjected to SDS PAGE followed by western blot with 12581-1-AP (UBE2Q2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IP experiment of HEK-293T using 12581-1-AP

    IP experiment of HEK-293T using 12581-1-AP

    IP result of anti-UBE2Q2 (IP:12581-1-AP, 4ug; Detection:12581-1-AP 1:300) with HEK-293T cells lysate 1320 ug.

经过测试的应用

Positive WB detected inA431 cells, mouse ovary tissue, HEK-293T cells
Positive IP detected inHEK-293T cells
Positive IHC detected inhuman skin cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

12581-1-AP targets UBE2Q2 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IP, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Immunogen UBE2Q2 fusion protein Ag3276 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ubiquitin-conjugating enzyme E2Q family member 2
Synonyms UBCi, UBE2Q2, Ubiquitin carrier protein Q2, Ubiquitin protein ligase Q2
Calculated Molecular Weight 375 aa, 43 kDa
Observed Molecular Weight 43 kDa
GenBank Accession NumberBC017708
Gene Symbol UBE2Q2
Gene ID (NCBI) 92912
RRIDAB_2256807
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8WVN8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for UBE2Q2 antibody 12581-1-APDownload protocol
IHC protocol for UBE2Q2 antibody 12581-1-APDownload protocol
IP protocol for UBE2Q2 antibody 12581-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Cell

In vivo structural characterization of the SARS-CoV-2 RNA genome identifies host proteins vulnerable to repurposed drugs.

Authors - Lei Sun
View Article
mouseWB

Transl Cancer Res

Proteome-wide identification and functional analysis of ubiquitinated proteins in Hepa1-6 cells by knockdown of E3 ubiquitin ligase SIAH1

Authors - Xiao Hu
View Article