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UBE2R2 Polyclonal antibody

UBE2R2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 14077-1-AP

Print datasheet

Synonyms

Ubiquitin protein ligase R2, Ubiquitin carrier protein R2, UBC3B, EC:2.3.2.23, E2 ubiquitin-conjugating enzyme R2

验证数据展示

  • WB analysis of Jurkat using 14077-1-AP

    WB analysis of Jurkat using 14077-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 14077-1-AP (UBE2R2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human spleen using 14077-1-AP

    IHC staining of human spleen using 14077-1-AP

    Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 14077-1-AP (UBE2R2 Antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human spleen using 14077-1-AP

    IHC staining of human spleen using 14077-1-AP

    Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 14077-1-AP (UBE2R2 Antibody) at dilution of 1:200 (under 40x lens).

  • IF Staining of HeLa using 14077-1-AP

    IF Staining of HeLa using 14077-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 14077-1-AP (UBE2R2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inJurkat cells, HeLa cells, mouse spleen tissue
Positive IHC detected inhuman spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 4 publications below

产品信息

14077-1-AP targets UBE2R2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Immunogen UBE2R2 fusion protein Ag5202 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ubiquitin-conjugating enzyme E2R 2
Synonyms Ubiquitin protein ligase R2, Ubiquitin carrier protein R2, UBC3B, EC:2.3.2.23, E2 ubiquitin-conjugating enzyme R2
Calculated Molecular Weight 27 kDa
Observed Molecular Weight 28-30 kDa
GenBank Accession NumberBC047584
Gene Symbol UBE2R2
Gene ID (NCBI) 54926
RRIDAB_2878010
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ712K3
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for UBE2R2 antibody 14077-1-APDownload protocol
IHC protocol for UBE2R2 antibody 14077-1-APDownload protocol
IF protocol for UBE2R2 antibody 14077-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

EMBO J

Shared and distinct mechanisms of UBA1 inactivation across different diseases

Authors - Jason C Collins
View Article
mouseWB

JCI Insight

PMID: 31292296

Authors - Isabelle K Vila
View Article
humanWB

bioRxiv

Shared and Distinct Mechanisms of UBA1 Inactivation Across Different Diseases

Authors - Jason C Collins
View Article
humanWB

Exp Gerontol

p53 accelerates endothelial cell senescence in diabetic retinopathy by enhancing FoxO3a ubiquitylation and degradation via UBE2L6

Authors - Ying Cheng
View Article