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  • KD/KO Validated

UBE2S Polyclonal antibody

UBE2S Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, ELISA and More (2)

Conjugate

Unconjugated

Cat no : 14115-1-AP

Print datasheet

Synonyms

OK/SW-cl.73, EPF5, EC:2.3.2.23, E2-EPF, E2EPF



经过测试的应用

Positive WB detected inMCF-7 cells, HEK-293T cells, MOLT-4 cells, PC-3 cells, HEK-293 cells, HeLa cells
Positive IP detected inA2780 cells
Positive IHC detected inhuman liver cancer tissue, human breast cancer tissue, human skin tissue, human ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14115-1-AP targets UBE2S in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IP, ELISA Application Description
Cited ApplicationsWB, IHC, IF, IP, CoIP
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Immunogen UBE2S fusion protein Ag5264 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ubiquitin-conjugating enzyme E2S
Synonyms OK/SW-cl.73, EPF5, EC:2.3.2.23, E2-EPF, E2EPF
Calculated Molecular Weight 222 aa, 24 kDa
Observed Molecular Weight 28 kDa
GenBank Accession NumberBC066948
Gene Symbol UBE2S
Gene ID (NCBI) 27338
RRIDAB_2256809
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ16763
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

UBE2S(Ubiquitin-conjugating enzyme E2 S) is also named as E2EPF and belongs to the ubiquitin-conjugating enzyme family. It acts as an essential factor of the anaphase promoting complex/cyclosome (APC/C) and a cell cycle-regulated ubiquitin ligase that controls progression through mitosis. Overexpression of UBE2S can increase tumor cell proliferation, invasion, and metastasis through the VHL-HIF pathway (PMID:16819549).

实验方案

Product Specific Protocols
WB protocol for UBE2S antibody 14115-1-APDownload protocol
IHC protocol for UBE2S antibody 14115-1-APDownload protocol
IP protocol for UBE2S antibody 14115-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

EMBO J

Weakened APC/C activity at mitotic exit drives cancer vulnerability to KIF18A inhibition

Authors - Colin R Gliech
  • KD Validated
mouseWB

Protein Cell

BMP4 preserves the developmental potential of mESCs through Ube2s- and Chmp4b-mediated chromosomal stability safeguarding.

Authors - Mingzhu Wang
  • KD Validated
humanWB,IHC

Int J Biol Sci

UBE2S as a novel ubiquitinated regulator of p16 and β-catenin to promote bone metastasis of prostate cancer.

Authors - Shengmeng Peng
  • KD Validated
humanWB

Int J Biol Sci

The Role of CHK1 Varies with the Status of Oestrogen-receptor and Progesterone-receptor in the Targeted Therapy for Breast Cancer.

Authors - Wei Xu
humanWB,IHC

J Ovarian Res

UBE2S promotes the progression and Olaparib resistance of ovarian cancer through Wnt/β-catenin signaling pathway.

Authors - Wenjing Hu
humanWB,IHC

Biochem Biophys Res Commun

UBE2S associated with OSCC proliferation by promotion of P21 degradation via the ubiquitin-proteasome system.

Authors - Shusaku Yoshimura
  • KD Validated