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UBE2S Polyclonal antibody

UBE2S Polyclonal Antibody for WB, IHC, IP, ELISA
Cat No. 14115-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IP, ELISA and More (2)

OK/SW-cl.73, EPF5, EC:2.3.2.23, E2-EPF, E2EPF

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inMCF-7 cells, HEK-293T cells, MOLT-4 cells, PC-3 cells, HEK-293 cells, HeLa cells
Positive IP detected inA2780 cells
Positive IHC detected inhuman liver cancer tissue, human breast cancer tissue, human skin tissue, human ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14115-1-AP targets UBE2S in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IP, ELISA Application Description
文献引用应用WB, IHC, IF, IP, CoIP
经测试反应性 human, mouse, rat
文献引用反应性human, mouse
免疫原 UBE2S fusion protein Ag5264 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ubiquitin-conjugating enzyme E2S
别名 OK/SW-cl.73, EPF5, EC:2.3.2.23, E2-EPF, E2EPF
计算分子量 222 aa, 24 kDa
观测分子量 28 kDa
GenBank蛋白编号BC066948
基因名称 UBE2S
Gene ID (NCBI) 27338
RRIDAB_2256809
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ16763
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

UBE2S(Ubiquitin-conjugating enzyme E2 S) is also named as E2EPF and belongs to the ubiquitin-conjugating enzyme family. It acts as an essential factor of the anaphase promoting complex/cyclosome (APC/C) and a cell cycle-regulated ubiquitin ligase that controls progression through mitosis. Overexpression of UBE2S can increase tumor cell proliferation, invasion, and metastasis through the VHL-HIF pathway (PMID:16819549).

实验方案

Product Specific Protocols
WB protocol for UBE2S antibody 14115-1-APDownload protocol
IHC protocol for UBE2S antibody 14115-1-APDownload protocol
IP protocol for UBE2S antibody 14115-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

EMBO J

Weakened APC/C activity at mitotic exit drives cancer vulnerability to KIF18A inhibition

Authors - Colin R Gliech
  • KD Validated
mouseWB

Protein Cell

BMP4 preserves the developmental potential of mESCs through Ube2s- and Chmp4b-mediated chromosomal stability safeguarding.

Authors - Mingzhu Wang
  • KD Validated
humanWB,IHC

Int J Biol Sci

UBE2S as a novel ubiquitinated regulator of p16 and β-catenin to promote bone metastasis of prostate cancer.

Authors - Shengmeng Peng
  • KD Validated
humanWB

Int J Biol Sci

The Role of CHK1 Varies with the Status of Oestrogen-receptor and Progesterone-receptor in the Targeted Therapy for Breast Cancer.

Authors - Wei Xu
humanWB,IHC

J Ovarian Res

UBE2S promotes the progression and Olaparib resistance of ovarian cancer through Wnt/β-catenin signaling pathway.

Authors - Wenjing Hu
humanWB,IHC

Biochem Biophys Res Commun

UBE2S associated with OSCC proliferation by promotion of P21 degradation via the ubiquitin-proteasome system.

Authors - Shusaku Yoshimura
  • KD Validated
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