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UBE2S Polyclonal antibody

UBE2S Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 18219-1-AP

Print datasheet

Synonyms

OK/SW-cl.73, EPF5, EC:2.3.2.23, E2-EPF, E2EPF

验证数据展示

  • WB analysis of HL-60 using 18219-1-AP

    WB analysis of HL-60 using 18219-1-AP

    HL-60 cells were subjected to SDS PAGE followed by western blot with 18219-1-AP (UBE2S antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • IHC staining of human skin cancer using 18219-1-AP

    IHC staining of human skin cancer using 18219-1-AP

    Immunohistochemical analysis of paraffin-embedded skin cancer slide using 18219-1-AP (UBE2S antibody) at dilution of 1:300 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human ovary cancer using 18219-1-AP

    IHC staining of human ovary cancer using 18219-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 18219-1-AP (UBE2S antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inHL-60 cells, COLO 320 cells, MCF-7 cells, MDA-MB-453s cells, mouse ovary tissue
Positive IHC detected inhuman skin cancer tissue, human ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:150-1:600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

18219-1-AP targets UBE2S in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen UBE2S fusion protein Ag12978 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ubiquitin-conjugating enzyme E2S
Synonyms OK/SW-cl.73, EPF5, EC:2.3.2.23, E2-EPF, E2EPF
Calculated Molecular Weight 24 kDa
Observed Molecular Weight 28 kDa
GenBank Accession NumberBC004236
Gene Symbol UBE2S
Gene ID (NCBI) 27338
RRIDAB_10598771
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ16763
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

UBE2S(Ubiquitin-conjugating enzyme E2 S) is also named as E2EPF and belongs to the ubiquitin-conjugating enzyme family. It acts as an essential factor of the anaphase promoting complex/cyclosome (APC/C) and a cell cycle-regulated ubiquitin ligase that controls progression through mitosis. Overexpression of UBE2S can increase tumor cell proliferation, invasion, and metastasis through the VHL-HIF pathway (PMID:16819549).

实验方案

Product Specific Protocols
WB protocol for UBE2S antibody 18219-1-APDownload protocol
IHC protocol for UBE2S antibody 18219-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols