• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis of HEK-293 using 66897-1-Ig

    WB analysis of HEK-293 using 66897-1-Ig

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 66897-1-Ig (UBE2S antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

UBE2S Monoclonal antibody

UBE2S Monoclonal Antibody for WB, ELISA
Cat No. 66897-1-Ig

Print datasheet

CloneNo. 8C9E6

Host / Isotype

Mouse / IgG2b

Reactivity

Human

Applications

WB, ELISA

Synonyms

E2 EPF, E2EPF, EPF5, UBE2S, Ubiquitin carrier protein S, Ubiquitin protein ligase S

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected inHEK-293 cells, Jurkat cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66897-1-Ig targets UBE2S in WB, ELISA applications and shows reactivity with Human samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity Human
Immunogen UBE2S fusion protein Ag5378 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name ubiquitin-conjugating enzyme E2S
Synonyms E2 EPF, E2EPF, EPF5, UBE2S, Ubiquitin carrier protein S, Ubiquitin protein ligase S
Calculated Molecular Weight 222 aa, 24 kDa
Observed Molecular Weight 28 kDa
GenBank Accession NumberBC066948
Gene Symbol UBE2S
Gene ID (NCBI) 27338
RRIDAB_2882226
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ16763
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

UBE2S(Ubiquitin-conjugating enzyme E2 S) is also named as E2EPF and belongs to the ubiquitin-conjugating enzyme family. It acts as an essential factor of the anaphase promoting complex/cyclosome (APC/C) and a cell cycle-regulated ubiquitin ligase that controls progression through mitosis. Overexpression of UBE2S can increase tumor cell proliferation, invasion, and metastasis through the VHL-HIF pathway (PMID:16819549).

实验方案

Product Specific Protocols
WB protocol for UBE2S antibody 66897-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols