验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, HepG2 cells, K-562 cells, Jurkat cells, SKOV-3 cells |
| Positive IP detected in | HeLa cells |
| Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
10105-2-AP targets UBE2T/HSPC150 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, IP |
| 经测试反应性 | human |
| 文献引用反应性 | human |
| 免疫原 | UBE2T/HSPC150 fusion protein Ag0153 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | ubiquitin-conjugating enzyme E2T (putative) |
| 别名 | HSPC150, UBE2T, PIG50, EC:2.3.2.23, E2 ubiquitin-conjugating enzyme T |
| 计算分子量 | 23 kDa |
| 观测分子量 | 23 kDa |
| GenBank蛋白编号 | BC004152 |
| 基因名称 | UBE2T |
| Gene ID (NCBI) | 29089 |
| RRID | AB_2211478 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9NPD8 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The ubiquitin (Ub)-mediated protein degradation pathway involves three sequential enzymatic steps that facilitate the conjugation of Ub to specific protein substrates. The first step requires ATP-dependent activation of the C-terminus of Ub and the assembly of multi-Ubs by Ub-activating enzyme E1. The ubiquitin-conjugating enzyme E2, catalytic (UBCc) domain, is then conjugated to Ubs, through a thiol-ester linkage between a conserved cysteine and the C-terminus of Ub, to generate an intermediate Ub-E2 complex. Then the E3, a ligase, catalyzes the transfer of Ub from E2 to the appropriate substrate. This pathway regulates many fundamental cellular processes. There are also other E2s which form thiol-ester linkages without the use of E3s as well as several UBC homologs (TSG101, Mms2, Croc-1 and similar proteins), which lack the active site cysteine essential for ubiquitination and appear to function in DNA repair pathways.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for UBE2T/HSPC150 antibody 10105-2-AP | Download protocol |
| IF protocol for UBE2T/HSPC150 antibody 10105-2-AP | Download protocol |
| IP protocol for UBE2T/HSPC150 antibody 10105-2-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Am J Hum Genet Mutations in the Gene Encoding the E2 Conjugating Enzyme UBE2T Cause Fanconi Anemia. | ||
Cancer Res Proteasome function is required for DNA damage response and fanconi anemia pathway activation. | ||
Blood Adv Amplification and overexpression of E2 ubiquitin conjugase UBE2T promotes homologous recombination in multiple myeloma. | ||
Oncotarget UBE2T promotes nasopharyngeal carcinoma cell proliferation, invasion, and metastasis by activating the AKT/GSK3β/β-catenin pathway.
| ||
Biomed Pharmacother Affinity-based protein profiling to reveal targets of puerarin involved in its protective effect on cardiomyocytes. | ||
Tumour Biol Ubiquitin-Conjugating Enzyme E2T is an Independent Prognostic Factor and Promotes Gastric Cancer Progression. |
