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UBE2V1 Polyclonal antibody

UBE2V1 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 10207-2-AP

Print datasheet

Synonyms

CIR1, CROC 1, CROC1, UBE2V, UBE2V1, UEV 1, UEV1, UEV1A

验证数据展示

  • WB analysis of human spleen using 10207-2-AP

    WB analysis of human spleen using 10207-2-AP

    human spleen tissue were subjected to SDS PAGE followed by western blot with 10207-2-AP (UBE2V1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inhuman spleen tissue, BxPC-3 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10207-2-AP targets UBE2V1 in WB, ELISA applications and shows reactivity with human samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity human
Immunogen UBE2V1 fusion protein Ag0278 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ubiquitin-conjugating enzyme E2 variant 1
Synonyms CIR1, CROC 1, CROC1, UBE2V, UBE2V1, UEV 1, UEV1, UEV1A
Calculated Molecular Weight 20 kDa
Observed Molecular Weight 20 kDa
GenBank Accession NumberBC000468
Gene Symbol UBE2V1
Gene ID (NCBI) 7335
RRIDAB_2272560
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ13404
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

UBE2V1(Ubiquitin-conjugating enzyme E2 variant 1) is also named as CROC1, UBE2V, UEV1 and belongs to the ubiquitin-conjugating enzyme family. It has no ubiquitin ligase activity on its own and the UBE2V1-UBE2N heterodimer catalyzes the synthesis of non-canonical poly-ubiquitin chains that are linked through Lys-63. CROC1 transcripts in all human tissues examined, with highest levels in brain, skeletal muscle, and kidney(PMID:9305758). It has 5 isoforms produced by alternative splicing.

实验方案

Product Specific Protocols
WB protocol for UBE2V1 antibody 10207-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols