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验证数据展示

  • WB analysis of human spleen using 10207-2-AP

    WB analysis of human spleen using 10207-2-AP

    human spleen tissue were subjected to SDS PAGE followed by western blot with 10207-2-AP (UBE2V1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

UBE2V1 Polyclonal antibody

UBE2V1 Polyclonal Antibody for WB, ELISA
Cat No. 10207-2-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, ELISA

别名

CIR1, CROC 1, CROC1, UBE2V, UBE2V1, UEV 1, UEV1, UEV1A

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inhuman spleen tissue, BxPC-3 cells
Planning an IHC experiment? We recommend our IHCeasy UBE2V1 Ready-To-Use IHC Kit. UBE2V1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:200-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10207-2-AP targets UBE2V1 in WB, ELISA applications and shows reactivity with human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human
免疫原 UBE2V1 fusion protein Ag0278 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ubiquitin-conjugating enzyme E2 variant 1
别名 CIR1, CROC 1, CROC1, UBE2V, UBE2V1, UEV 1, UEV1, UEV1A
计算分子量 20 kDa
观测分子量 20 kDa
GenBank蛋白编号BC000468
基因名称 UBE2V1
Gene ID (NCBI) 7335
RRIDAB_2272560
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ13404
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

UBE2V1(Ubiquitin-conjugating enzyme E2 variant 1) is also named as CROC1, UBE2V, UEV1 and belongs to the ubiquitin-conjugating enzyme family. It has no ubiquitin ligase activity on its own and the UBE2V1-UBE2N heterodimer catalyzes the synthesis of non-canonical poly-ubiquitin chains that are linked through Lys-63. CROC1 transcripts in all human tissues examined, with highest levels in brain, skeletal muscle, and kidney(PMID:9305758). It has 5 isoforms produced by alternative splicing.

实验方案

Product Specific Protocols
WB protocol for UBE2V1 antibody 10207-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols