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MMS2 Polyclonal antibody

MMS2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 10689-1-AP

Print datasheet

Synonyms

UBE2V2, EDPF1, EDPF 1, EDAF-1, EDAF 1

验证数据展示

  • WB analysis of HEK-293 using 10689-1-AP

    WB analysis of HEK-293 using 10689-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 10689-1-AP (MMS2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 10689-1-AP

    IF Staining of HeLa using 10689-1-AP

    Immunofluorescent analysis of HeLa cells using 10689-1-AP (MMS2 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IHC staining of human colon using 10689-1-AP

    IHC staining of human colon using 10689-1-AP

    Immunohistochemical analysis of paraffin-embedded human normal colon slide using 10689-1-AP (MMS2 antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inHEK-293 cells, A375 cells, HeLa cells, HL-60 cells, human brain tissue, Jurkat cells, K-562 cells, rat liver tissue
Positive IHC detected inhuman colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells, HEK-293 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:400-1:1600
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10689-1-AP targets MMS2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Cited ApplicationsWB, IF
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Immunogen MMS2 fusion protein Ag1111 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ubiquitin-conjugating enzyme E2 variant 2
Synonyms UBE2V2, EDPF1, EDPF 1, EDAF-1, EDAF 1
Calculated Molecular Weight 16 kDa
Observed Molecular Weight 16-20 kDa
GenBank Accession NumberBC007051
Gene Symbol MMS2
Gene ID (NCBI) 7336
RRIDAB_2211656
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ15819
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for MMS2 antibody 10689-1-APDownload protocol
IHC protocol for MMS2 antibody 10689-1-APDownload protocol
IF protocol for MMS2 antibody 10689-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

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