验证数据展示
经过测试的应用
| Positive WB detected in | K-562 cells, mouse brain tissue, mouse lung tissue, Jurkat cells, NIH/3T3 cells, C6 cells |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | human thyroid cancer tissue, human kidney tissue, mouse lung tissue, rat liver tissue, mouse kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:300-1:1200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
10344-1-AP targets UBE3A in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, IP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 | UBE3A fusion protein Ag0346 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | ubiquitin protein ligase E3A |
| 别名 | E6AP ubiquitin-protein ligase, E6AP ubiquitin protein ligase, E6AP, E6 AP, AS |
| 计算分子量 | 852 aa, 98 kDa |
| 观测分子量 | 100 kDa |
| GenBank蛋白编号 | BC002582 |
| 基因名称 | UBE3A |
| Gene ID (NCBI) | 7337 |
| RRID | AB_2211801 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q05086 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
UBE3A(Ubiquitin-protein ligase E3A) is also named as E6AP, EPVE6AP, HPVE6A and belongs to the E3 ubiquitin protein ligase family. It functions as both an E3 ligase in the ubiquitin proteasome pathway and as a transcriptional coactivator. It is also initially identified as a cellular protein that mediates in vitro association of the human papillomavirus E6 protein with p53, leading to the ubiquitin-dependent degradation of p53(PMID:1661671). Defects in UBE3A are a cause of Angelman syndrome (AS)(PMID:10508479). It has 3 isoforms with MW of 97-101 kDa produced by alternative splicing.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for UBE3A antibody 10344-1-AP | Download protocol |
| IHC protocol for UBE3A antibody 10344-1-AP | Download protocol |
| IF protocol for UBE3A antibody 10344-1-AP | Download protocol |
| IP protocol for UBE3A antibody 10344-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Mol Cell Biol Landscape of genomic imprinting and its functions in the mouse mammary gland. | ||
Oncogene Celastrol recruits UBE3A to recognize and degrade the DNA binding domain of steroid receptors
| ||
Cell Death Discov SNORD6 promotes cervical cancer progression by accelerating E6-mediated p53 degradation | ||
Int J Biol Sci UBE3A activates the NOTCH pathway and promotes esophageal cancer progression by degradation of ZNF185.
| ||
Mol Neurobiol All-Trans Retinoic Acid Exerts Neuroprotective Effects in Amyotrophic Lateral Sclerosis-Like Tg (SOD1*G93A)1Gur Mice. |
