• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

UBE3A Monoclonal antibody

UBE3A Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

3D6H9

Cat no : 60038-1-Ig

Print datasheet

Synonyms

ANCR, AS, E6 AP, E6AP, E6AP ubiquitin protein ligase, EPVE6AP, HPVE6A, UBE3A, ubiquitin protein ligase E3A

验证数据展示

  • WB analysis of HeLa using 60038-1-Ig

    WB analysis of HeLa using 60038-1-Ig

    HeLa cells were subjected to SDS PAGE followed by western blot with 60038-1-Ig (UBE3A antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human kidney using 60038-1-Ig

    IHC staining of human kidney using 60038-1-Ig

    Immunohistochemical analysis of paraffin-embedded human kidney using 60038-1-Ig(UBE3A antibody) at dilution of 1:50 (under 10x lens).

    Ready-to-use IHC kits available with UBE3A antibody included
  • IHC staining of human kidney using 60038-1-Ig

    IHC staining of human kidney using 60038-1-Ig

    Immunohistochemical analysis of paraffin-embedded human kidney using 60038-1-Ig(UBE3A antibody) at dilution of 1:50 (under 40x lens).

    Ready-to-use IHC kits available with UBE3A antibody included

经过测试的应用

Positive WB detected inHeLa cells
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy UBE3A Ready-To-Use IHC Kit. UBE3A primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:400
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

60038-1-Ig targets UBE3A in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse
Immunogen UBE3A fusion protein Ag0346 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name ubiquitin protein ligase E3A
Synonyms ANCR, AS, E6 AP, E6AP, E6AP ubiquitin protein ligase, EPVE6AP, HPVE6A, UBE3A, ubiquitin protein ligase E3A
Calculated Molecular Weight 852 aa, 98 kDa
Observed Molecular Weight 100 kDa
GenBank Accession NumberBC002582
Gene Symbol UBE3A
Gene ID (NCBI) 7337
Conjugate Unconjugated
Form Liquid
Purification MethodCaprylic acid/ammonium sulfate precipitation
UNIPROT IDQ05086
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

UBE3A(Ubiquitin-protein ligase E3A) is also named as E6AP, EPVE6AP, HPVE6A and belongs to the E3 ubiquitin protein ligase family. It functions as both an E3 ligase in the ubiquitin proteasome pathway and as a transcriptional coactivator. It is also initially identified as a cellular protein that mediates in vitro association of the human papillomavirus E6 protein with p53, leading to the ubiquitin-dependent degradation of p53(PMID:1661671). Defects in UBE3A are a cause of Angelman syndrome (AS)(PMID:10508479 ). It has 3 isoforms produced by alternative splicing.