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UBE3C Polyclonal antibody

UBE3C Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 12333-1-AP

Print datasheet

Synonyms

KIAA10, KIAA0010, Homologous to E6AP carboxyl terminus homologous protein 2, HECT-type ubiquitin transferase E3C, HectH2

验证数据展示

  • WB analysis using 12333-1-AP

    WB analysis using 12333-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12333-1-AP (UBE3C antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • IHC staining of human stomach using 12333-1-AP

    IHC staining of human stomach using 12333-1-AP

    Immunohistochemical analysis of paraffin-embedded human normal stomach slide using 12333-1-AP (UBE3C antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inHepG2 cells, mouse liver tissue, rat liver tissue
Positive IHC detected inhuman stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

12333-1-AP targets UBE3C in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Immunogen UBE3C fusion protein Ag2966 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ubiquitin protein ligase E3C
Synonyms KIAA10, KIAA0010, Homologous to E6AP carboxyl terminus homologous protein 2, HECT-type ubiquitin transferase E3C, HectH2
Calculated Molecular Weight 124 kDa
Observed Molecular Weight 70 kDa
GenBank Accession NumberBC026241
Gene Symbol UBE3C
Gene ID (NCBI) 9690
RRIDAB_2877846
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ15386
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for UBE3C antibody 12333-1-APDownload protocol
IHC protocol for UBE3C antibody 12333-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
human,mouseWB

Int Dent J

LncRNA AFAP1-AS1 Promotes Oral Squamous Cell Carcinoma Development by Ubiquitin-Mediated Proteolysis

Authors - Bao-Jun Li
View Article